Mercury chronically release from dental amalgam fillings are known could represent a continuous source of oxidative damage to mouth tissues. Previous study showed that the correlation between salivary total antioxidant activity and salivary mercury levels was not significant in males while on the other hand was significant in females. Objective: The present study was aimed at verifying the hypothesis by comparing the salivary malondialdehyde (MDA) and reduced glutathione (GSH) levels between amalgam and non-amalgam fillings subjects. Methods: Samples of saliva from 10 healthy without amalgam fillings and 10 healthy with amalgam fillings were analyzed for MDA and GSH using spectrophotometer method. Results: The results indicated that salivary MDA levels in amalgam free subjects (0.089±0.047 nmol mL-1) was significantly higher (p=0.019) than salivary MDA levels in subjects with amalgam (0.045±0.011 nmol mL-1). However, the salivary GSH levels in amalgam free subjects and subjects with amalgam fillings was not different (0.82±0.39 nmol mL-1 and 1.02±0.019 nmol mL-1) respectively. Conclusions: It was concluded that salivary MDA levels can be used as indicator of salivary antioxidant activity influenced by the pro-oxidant role of amalgam mercury.