Objective: In Indonesia, traditionally, the extract of Saccharum spontaneum L. has been used for tooth whitening and to ease the removal of calculus. The mechanism based on previous research of tooth whitening and removal of calculus by certain chemical of actions, such as superooxyde and some acids as miris, and glutamic acid and other acids as a-hydroxyl acid such palmitate and acetic acid which has been detected. Methods: Hence a study was carried out to learn the safety level of Saccharum spontaneum L. in vitro as well as to understand the life cycle of the cells after being treated, and the control of this study was H2O2 3%. After harvesting the fibroblast BHK-21 clone 13 cell line, dropped 1-2 of trypsin versene and centrifuges twice and than continued by treated with trizol, which consist of 0.8M guanidine thiocianate, 0.1M ammonium thiocianate, 0.1M sodium acetate in 5% glycerol and 38% of phenol. The electrophoresis with composite gel is then being run after 5 minutes of incubation to detect DNA bands of the cell line. Results: This study showed that DNA bands of the cells which it has been treated with fractions of the Saccharum spontaneum L. are in the same bands with the fibroblast BHK-21 clone 13 cell lines, and there is no evidence of cell lysis. On the other hand, all cells which have been treated using H2O2 3% were lysis. Conclusions: This study showed that the cells treated with the extract of Saccharum spontaneum L. are safe to fibroblast BHK-21 cell line and other livelihood cells, which showed good proliferated as cells while treating.