Squid pen from Loligo opalescens, a much opened porosity, is a rich source of â-chitin and chitosan. These substances were wound healing promoting agent. Objective: To examine the osteogenic properties of squid pen on mouse osteoblasts cell line (MC3T3-E1). Method: MC3T3-E1 cells were cultured in 24-well plate at a density of 2,000 cells/well in α-MEM complete medium. Culture was treated with small pieces of squid pen in experimental group and fed in complete medium as control group. The mineralized nodule was stained with Alizarin red S and morphological feature was studied with scanning electron microscopy (SEM). Results: The study showed that after 3 weeks of treatment, mineralized nodules were obviously observed in the experimental group. Moreover, MC3T3-E1cells accumulated closely to each piece of squid pen. Phase contrast and SEM examination revealed heavily colonized cells on surface of squid pen with some cells scattered on its porous surface. Conclusion: The osteoinductive and osteoconductive properties without cytotoxicity of squid pen might be useful to develop this biocompatible material as scaffold for bone tissue engineering.