IADR Abstract Archives
Topical Fluoride-Varnish Shifts Dysbiotic Plaque Microbiome towards Eubiosis in Children
Objectives: Fluoride varnish (FV) has been widely used as a caries-inhibitory agent to reduce the risk of caries development. FV application was found to significantly inhibit early childhood caries and reverse demineralization due to disturbances in dental plaque microbiology. This study aimed to investigate the effect of sustained fluoride release on the microbial diversity of dental plaque biofilms between 8-9-year-old children with mixed dentition using 16S rRNA gene sequencing.
Methods: Dental plaque samples collected from a clinical trial on caries in children were examined. The study population comprised 20 children aged 8–9 years who were diagnosed of having dental caries. FV was applied 1 month (once a week) to all subjects. Clinical parameters and demographic information of the subjects such as caries index (DMFT and dmft) were recorded at the baseline, salivary pH level and PHP index (Patient Hygiene Performance) were recorded at both baseline and after one month of the topical application of the FV. The plaque samples were collected both prior to (baseline) and after (one month) the topical application of the FV. Microbial diversity was further analyzed using 16S rRNA gene Next Generation Sequencing.
Results: After one month of FV application, the plaque microbiome has significantly shifted along with rare taxonomic for 37% (p < 0.01). Approximately 73% of the species that were significantly increased belong to the phylum Proteobacteria. It was shown that the significant differences in the composition dental plaque were caused by the reduction dental caries bacteria.
Conclusions: FV application led to a shift dental plaque biofilm from dysbiosis to eubiosis. Furthermore, weekly FV application for one month noticeably depleted certain established caries-causing bacteria while promoting the growth of rare, abundant genera primarily belonging to the Proteobacteria phylum, which may play a protective role in the progression of dental caries.
Methods: Dental plaque samples collected from a clinical trial on caries in children were examined. The study population comprised 20 children aged 8–9 years who were diagnosed of having dental caries. FV was applied 1 month (once a week) to all subjects. Clinical parameters and demographic information of the subjects such as caries index (DMFT and dmft) were recorded at the baseline, salivary pH level and PHP index (Patient Hygiene Performance) were recorded at both baseline and after one month of the topical application of the FV. The plaque samples were collected both prior to (baseline) and after (one month) the topical application of the FV. Microbial diversity was further analyzed using 16S rRNA gene Next Generation Sequencing.
Results: After one month of FV application, the plaque microbiome has significantly shifted along with rare taxonomic for 37% (p < 0.01). Approximately 73% of the species that were significantly increased belong to the phylum Proteobacteria. It was shown that the significant differences in the composition dental plaque were caused by the reduction dental caries bacteria.
Conclusions: FV application led to a shift dental plaque biofilm from dysbiosis to eubiosis. Furthermore, weekly FV application for one month noticeably depleted certain established caries-causing bacteria while promoting the growth of rare, abundant genera primarily belonging to the Proteobacteria phylum, which may play a protective role in the progression of dental caries.