IADR Abstract Archives
Dental Pulp Blood Vessel-Like Structures Response to Silver Diamine Fluoride
Objectives: This study aims to evaluate the response of the dental pulp blood vessel-like structures formed in a 3D cell culture to silver diamine fluoride treatment.
Methods: 3D spheres of dental pulp stem cells (DPSC) and human umbilical vein endothelial cells (HUVEC) with the ratio 3:1 were formed using a low attachment agarose mold. The spheres were left to grow for 14 days before treatment. The 3D spheres were treated with 0.0001% SDF (Group SDF 10X), 0.00001% SDF (Group SDF 1X), and no treatment (Group Control), respectively. The concentration was determined according to concentration of the SDF the in dental pulp when used as an indirect pulp capping agent in deep cavities. Live/Dead assay was performed. Viability was evaluated using CCK-8 assay.
Results: The average values of live/dead assay were 98.3% for Group SDF 10X, 98% for Group SDF 1X, and 96% for Group Control. There was no significant difference among the groups (p=0.378). The average values of the CCK-8 assay was 94% for Group SDF 10X, 87% for Group SDF 1X and 87% for Group Control. Group SDF 10X showed a significant higher cell viability than Group SDF 10X and Group Control (p<0.001)
Conclusions: SDF at a low concentration did not have an adverse effect on the dental pulp blood vessel-like structures. Moreover, 0.0001% SDF treatment increased the viability of the dental pulp cells.
Methods: 3D spheres of dental pulp stem cells (DPSC) and human umbilical vein endothelial cells (HUVEC) with the ratio 3:1 were formed using a low attachment agarose mold. The spheres were left to grow for 14 days before treatment. The 3D spheres were treated with 0.0001% SDF (Group SDF 10X), 0.00001% SDF (Group SDF 1X), and no treatment (Group Control), respectively. The concentration was determined according to concentration of the SDF the in dental pulp when used as an indirect pulp capping agent in deep cavities. Live/Dead assay was performed. Viability was evaluated using CCK-8 assay.
Results: The average values of live/dead assay were 98.3% for Group SDF 10X, 98% for Group SDF 1X, and 96% for Group Control. There was no significant difference among the groups (p=0.378). The average values of the CCK-8 assay was 94% for Group SDF 10X, 87% for Group SDF 1X and 87% for Group Control. Group SDF 10X showed a significant higher cell viability than Group SDF 10X and Group Control (p<0.001)
Conclusions: SDF at a low concentration did not have an adverse effect on the dental pulp blood vessel-like structures. Moreover, 0.0001% SDF treatment increased the viability of the dental pulp cells.