Pg-LPS increases proliferation, MMP-1 and IL-8 in cisplatin-resistance HSC-3 carcinoma

Objectives: The aim of this research is to investigate the effect of Pg-LPS on proliferation, matrix metalloproteinase-1 (MMP-1) activity, and interleukin-8 (IL-8) production of cisplatin-resistant human tongue squamous carcinoma HSC-3 cell line.
Methods: Human tongue squamous carcinoma, HSC-3 cells, were induced to become cisplatin-resistant (HSC-3/R). HSC-3 and HSC-3/R cells were cultured separately. The proliferative activity, MMP-1 activity and IL-8 production of both cell lines with and without Pg-LPS activation (0.25 - 1 μg/ml) were evaluated using MTT assays, gelatin zymography and ELISA, respectively.
Results: At 24 hours, Pg-LPS activated HSC-3 cells showed lower cell proliferation when compared to the control (P<0.05), whereas Pg-LPS activated HSC-3/R cells showed greater proliferation when compared to the control (P<0.05). Gelatin zymography illustrated that Pg-LPS activated HSC-3/R cells portrayed more MMP-1 production than HSC-3 group at 48 and 72 hours. In concordance with ELISA assay, the production of IL-8 was higher significantly in Pg-LPS activated HSC-3/R cells than that of HSC-3 at the same concentration (P<0.05).
Conclusions: The ability of cell proliferation, MMP-1 activity and IL-8 production in HSC-3/R cells once activated with Pg-LPS were higher than the parental HSC-3 cells. Hence, cisplatin-resistant carcinoma cell has increased aggressiveness in inflammatory condition.