IADR Abstract Archives
Injectable Polyxylitol Succinate-based Adhesive Hydrogel for Periodontal Regeneration
Objectives: To develop an injectable adhesive hydrogel with antibacterial and osteogenic properties for periodontal regeneration.
Methods: A novel copolymer polyxylitol succinate (PXS) was prepared through the esterification of xylitol with succinyl chloride. Adhesive properties were subsequently incorporated into the prepolymer by combining biocompatible catechols, via caffeic acid (CFA). The PXS and CFA were subjected to citric acid in a one-pot synthesis. The final injectable caffeic acid/PXS/citric acid composite polymer (iCPC) hydrogel was fabricated by cross-linking the pre-polymer solution with MgO. PXS and iCPC polymers were characterized by proton nuclear magnetic resonance (1H NMR) spectra. Adhesion strengths were examined by lap shear testing. Biocompatibility of iCPC on human periodontal ligament cells (hPDLCs) was examined by CCK8-assay and live/dead staining. Anti-bacterial effects of iCPC against Actinomycis actinomycetemcomitans and Porphyromonas gingivalis were examined by OD600 value recording. Alizarin red staining and alkaline phosphatase (ALP) activity of hPDLCs/iCPC were performed to assess the osteogenic properties. Statistical analysis was performed using one-way ANOVA and Tukey multiple comparison tests.
Results: The 1H NMR spectrum of PXS featured specific peaks corresponding to the methine protons from xylitol and the methylene protons of succinic acid. In iCPC polymer, specific peaks in the chemical shifts, which can be ascribed to CFA, confirmed the successful conjugation of CFA to the polymer backbone. Lap shear adhesion test results showed superior adhesive properties of iCPC compared to fibrin glue (P<0.05). CCK8 assays and live/dead staining results indicated that iCPC hydrogels are biocompatible and could enhance the proliferation of hPDLCs. OD600 values showed that iCPC hydrogel inhibited the growth of A. actinomycetemcomitans, and P. gingivalis confirming its anti-bacterial properties (P<0.05). Alizarin red staining for mineralized nodule formation and ALP activity results confirmed the superior osteogenic properties of iCPC hydrogels on hPDLCs (P<0.05).
Conclusions: iCPC hydrogel possesses excellent tissue-adhesive, antibacterial, and osteogenic properties required for periodontal regeneration.
Methods: A novel copolymer polyxylitol succinate (PXS) was prepared through the esterification of xylitol with succinyl chloride. Adhesive properties were subsequently incorporated into the prepolymer by combining biocompatible catechols, via caffeic acid (CFA). The PXS and CFA were subjected to citric acid in a one-pot synthesis. The final injectable caffeic acid/PXS/citric acid composite polymer (iCPC) hydrogel was fabricated by cross-linking the pre-polymer solution with MgO. PXS and iCPC polymers were characterized by proton nuclear magnetic resonance (1H NMR) spectra. Adhesion strengths were examined by lap shear testing. Biocompatibility of iCPC on human periodontal ligament cells (hPDLCs) was examined by CCK8-assay and live/dead staining. Anti-bacterial effects of iCPC against Actinomycis actinomycetemcomitans and Porphyromonas gingivalis were examined by OD600 value recording. Alizarin red staining and alkaline phosphatase (ALP) activity of hPDLCs/iCPC were performed to assess the osteogenic properties. Statistical analysis was performed using one-way ANOVA and Tukey multiple comparison tests.
Results: The 1H NMR spectrum of PXS featured specific peaks corresponding to the methine protons from xylitol and the methylene protons of succinic acid. In iCPC polymer, specific peaks in the chemical shifts, which can be ascribed to CFA, confirmed the successful conjugation of CFA to the polymer backbone. Lap shear adhesion test results showed superior adhesive properties of iCPC compared to fibrin glue (P<0.05). CCK8 assays and live/dead staining results indicated that iCPC hydrogels are biocompatible and could enhance the proliferation of hPDLCs. OD600 values showed that iCPC hydrogel inhibited the growth of A. actinomycetemcomitans, and P. gingivalis confirming its anti-bacterial properties (P<0.05). Alizarin red staining for mineralized nodule formation and ALP activity results confirmed the superior osteogenic properties of iCPC hydrogels on hPDLCs (P<0.05).
Conclusions: iCPC hydrogel possesses excellent tissue-adhesive, antibacterial, and osteogenic properties required for periodontal regeneration.