IADR Abstract Archives
Title: Characterization of human mesenchymal stem cells in cobalt-incorporated hydroxyapatite media
Objectives: Background: Cobalt-incorporated hydroxyapatite (HACo) has been developed for bone substitute material. Co treatment initiates hypoxia mimic to cells such as osteoclasts and osteoblasts. Co ions could be released from HACo. Therefore, the objective of this study was to determine the effects of Co release from HACo discs on human mesenchymal stem cells (hMSCs).
Methods: Materials and methods: HACo discs at concentrations 40, 400, 4,000, and 8,000 µM were fabricated by simple soaking method. HA was served as control. HA and HACo discs were incubated in DMEM media (conditioned media) for 3 days. The conditioned media were then collected and used for cell cultured. After conditioned medium treatment, cell adhesion, morphology, and time-lapse migration assay were performed.
Results: Results: Conditioned media collected from moderate concentration tended to increase number of cell adhesion. HACo enhanced cell spreading area and density of actin filament cytoskeleton. Time-lapse imaging showed that wound closure progressed continuously in every sample. After 18 hours, the highest migration of cells was seen in cells exposed to HACo40-conditioned media. The wound area in HACo40 was 47% of the area at time 0.
Conclusions: Conclusion: Co altered cell behaviors such as cell adhesion, cell morphology, and cell migration.
Methods: Materials and methods: HACo discs at concentrations 40, 400, 4,000, and 8,000 µM were fabricated by simple soaking method. HA was served as control. HA and HACo discs were incubated in DMEM media (conditioned media) for 3 days. The conditioned media were then collected and used for cell cultured. After conditioned medium treatment, cell adhesion, morphology, and time-lapse migration assay were performed.
Results: Results: Conditioned media collected from moderate concentration tended to increase number of cell adhesion. HACo enhanced cell spreading area and density of actin filament cytoskeleton. Time-lapse imaging showed that wound closure progressed continuously in every sample. After 18 hours, the highest migration of cells was seen in cells exposed to HACo40-conditioned media. The wound area in HACo40 was 47% of the area at time 0.
Conclusions: Conclusion: Co altered cell behaviors such as cell adhesion, cell morphology, and cell migration.