IADR Abstract Archives
Lactobacillus-reuteri modulates virulence gene of dental plaque on orthodontic patients
Objectives: Patients on fixed orthodontic appliances often have higher accumulation of dental plaque biofilms leading to microbial dysbiosis and oral pathologies. Probiotic bacteria is known to modulate the microbial dysbiosis. The aim of this study was to determine the efficacy of probiotic bacteria Lactobacillus reuteri containing lozenges on pathogenic trait of major oral pathogens Candida albicans and Fusobacterium nucleatum from dental plaque samples of patients wearing fixed orthodontic appliances
Methods: The subjects wearing fixed orthodontic appliances (n=20) enrolled to this prospective clinical trial consumed L. reuteriprobiotic lozenges (2 x 108 CFU/ml) each day for two weeks. Plaque samples were obtained using standard methodology were subjected to RNA extraction and cDNA synthesis. The expression of virulence-associated genes of C. albicans namely BCR1 and ACE2 and F. nucleatum namely fadA and aid1 were evaluated using qPCR. The data were statistically analyzed with the Shapiro-Wilk normality test and paired t-test2 where appropriate.
Results: The post-intervention with probiotic lozenges containing L. reuteri significantly downregulated the C. albicans BCR1 and ACE2 gene expression as well as fadA and aid1 gene expression in F. nucleatum in dental plaque biofilm samples of orthodontic patients (p < 0.05)
Conclusions: L. reuteri probiotic containing lozenges reduce the pathogenic-trait associated gene expression of key oral pathogens in dental plaque biofilms. This indicates that probiotic may bring beneficial effect to modulate dysbiotic dental biofilm commonly seen in patients wearing fixed orthodontic treatment.
Methods: The subjects wearing fixed orthodontic appliances (n=20) enrolled to this prospective clinical trial consumed L. reuteriprobiotic lozenges (2 x 108 CFU/ml) each day for two weeks. Plaque samples were obtained using standard methodology were subjected to RNA extraction and cDNA synthesis. The expression of virulence-associated genes of C. albicans namely BCR1 and ACE2 and F. nucleatum namely fadA and aid1 were evaluated using qPCR. The data were statistically analyzed with the Shapiro-Wilk normality test and paired t-test2 where appropriate.
Results: The post-intervention with probiotic lozenges containing L. reuteri significantly downregulated the C. albicans BCR1 and ACE2 gene expression as well as fadA and aid1 gene expression in F. nucleatum in dental plaque biofilm samples of orthodontic patients (p < 0.05)
Conclusions: L. reuteri probiotic containing lozenges reduce the pathogenic-trait associated gene expression of key oral pathogens in dental plaque biofilms. This indicates that probiotic may bring beneficial effect to modulate dysbiotic dental biofilm commonly seen in patients wearing fixed orthodontic treatment.