Potential Antibacterial Composite Resin Infused with Hydrated CaO
Objectives: Composite resin is one of the most promising restorative materials today. It is often associated with superior esthetic qualities, ease of use and a conservative approach towards cavity preparation. However, microorganisms accumulate more on the surface of this restoration than other restorative materials which causes secondary caries and eventually leads to failed restorations. The current study aimed to address this drawback by incorporating hydrated calcium oxide (CaO) from calcined Asian moon scallop (Amusium pleuronectes) shells to commercially-available composite resin to produce an antibacterial property. Methods: One control group and three experimental groups (composite containing 1%, 5% and 10% hydrated CaO) were utilized. Kirby-Bauer disk diffusion susceptibility test was conducted to evaluate the antibacterial property of the groups against gram-positive cocci. Furthermore, the properties of the composite resin, specifically the flexural strength, depth of cure, water sorption and solubility were also assessed to determine if these were compromised upon the addition of hydrated CaO. Shapiro-Wilk Test, Independent Samples t-Test and Mann-Whitney U test were used for data interpretation. Results: The experimental groups with hydrated CaO significantly inhibited the growth of gram-positive cocci having p values of 0.015, 0.020 and 0.027 respectively when compared to the control group. These results favor the experimental groups. Moreover, there was no significant difference on the flexural strength, depth of cure, water sorption and solubility of the experimental groups and control group except that of the 5% concentration which had better polymerization (p=0.034) and the 10% concentration which was more water soluble (p=0.046). Conclusions: It can be concluded that composite resin with hydrated CaO can be a promising restorative material with antibacterial efficacy and largely uncompromised properties. This innovation, alongside further research, can prevent the occurrence of failed restorations due to secondary caries formation.
2023 South East Asian Division Meeting (Singapore) Singapore
2023 102 Dental Materials 2: Polymer-based Materials
Canlas, Bn. Lucky
( Centro Escolar University
, Manila
, Manila
, Philippines
)
Lacanienta, Mary Iodine
( Centro Escolar University
, Manila
, Manila
, Philippines
)
Barongan, Princess Kyla Laine
( Centro Escolar University
, Manila
, Manila
, Philippines
)
Catapangan, Natalia Paola
( Centro Escolar University
, Manila
, Manila
, Philippines
)
Mendoza, Gwyneth Mari
( Centro Escolar University
, Manila
, Manila
, Philippines
)
Algas, Hannah Karla
( Centro Escolar University
, Manila
, Manila
, Philippines
)
Mendoza, Harren
( Centro Escolar University
, Manila
, Manila
, Philippines
)
Atrero, Marithe
( Centro Escolar University
, Manila
, Manila
, Philippines
)
Mendoza, Andrea Denise
( Centro Escolar University
, Manila
, Manila
, Philippines
)
Andres, Maricar Joy
( Centro Escolar University
, Manila
, Manila
, Philippines
)
Table 1. Comparison of Antibacterial Efficacy of the Control and Experimental Groups
Comparisons
Mean (mm)
z
p-value
Decision on H0
Interpretation
Control
0.000
8.000
0.015*
Reject
Significant
1% hydrated CaO
4.000
Control
0.000
7.000
0.020*
Reject
Significant
5% hydrated CaO
4.667
Control
0.000
6.000
0.027*
Reject
Significant
10% hydrated CaO
6.000
As depicted in Table 1, there is a significant difference between the control and experimental groups. The finding signifies that the composite with 1%, 5% and 10% hydrated CaO have an antibacterial property while the control group has none.
Table 2. Comparison of the Physical and Mechanical Properties of the Control and Experimental Groups
Properties
Comparisons
Mean
z
p-value
Decision on H0
Interpretation
Flexural Strength
Control
71.754 MPa
0.218
0.827
Accept
Not Significant
1% Hydrated CaO
59.417 MPa
Control
71.754 MPa
0.655
0.513
Accept
Not Significant
5% Hydrated CaO
45.161 MPa
Control
71.754 MPa
1.528
0.127
Accept
Not Significant
10% Hydrated CaO
40.092 MPa
Depth of Cure
Control
4.900 mm
1.000
0.317
Accept
Not Significant
1% Hydrated CaO
4.950 mm
Control
4.900 mm
2.121
0.034*
Reject
Significant
5% Hydrated CaO
5.000 mm
Control
4.900 mm
1.650
0.099
Accept
Not Significant
10% Hydrated CaO
4.983 mm
Water Sorption
Control
0.00001321 g/mm3
1.291
0.197
Accept
Not Significant
1% Hydrated CaO
0.00000943 g/mm3
Control
0.00001321 g/mm3
0.471
0.637
Accept
Not Significant
5% Hydrated CaO
0.00000755 g/mm3
Control
0.00001321 g/mm3
1.650
0.099
Accept
Not Significant
10% Hydrated CaO
0.00001887 g/mm3
Water Solubility
Control
0.00000755 g/mm3
0.745
0.456
Accept
Not Significant
1% Hydrated CaO
0.00000943 g/mm3
Control
0.00000755 g/mm3
0.000
1.000
Accept
Not Significant
5% Hydrated CaO
0.00000755 g/mm3
Control
0.00000755 g/mm3
1.993
0.046*
Reject
Significant
10% Hydrated CaO
0.00002264 g/mm3
Table 2 shows that there is no significant difference between the control and experimental groups in terms of flexural strength, depth of cure, water sorption and water solubility except that of the 5% hydrated CaO which polymerized better and the 10% hydrated CaO which became more soluble. The results reveal that the addition of hydrated CaO to composite resin did not compromise the majority of its properties.