IADR Abstract Archives
Plant-Derived Irrigation Solutions: Effect on Dentin Microhardness and Smear Layer.
Objectives: Several plant extracts have shown promise when used as irrigants or medicaments during root canal treatment due to their antimicrobial, anti-inflammatory, and therapeutic effects. This study aims to compare the effects of three plant-derived root canal irrigants, namely cat whiskers, clove and ginger, on dentine microhardness and smear layer removal.
Methods: Eighty-four extracted permanent single-rooted premolars were selected. Microhardness analysis was conducted using Vickers indenter on sixty decoronated roots. Root canal preparation was carried out prior to longitudinal resection of the root specimens and immersion for 5 minutes in six experimental and control solutions (n=10), namely: Group 1: 0.5 % ginger, Group 2: 2.5 % cat whiskers, Group 3: 0.05 % clove, Group 4: 2.5 % sodium hypochlorite (NaOCl), Group 5: 17 % ethylenediaminetetraacetic acid (EDTA) and Group 6: 0.9 % normal saline. Microhardness values were determined before and after immersion. Smear layer removal (n=24) at the coronal middle and apical root third was analysed using field-emission scanning electron microscopy analysis (FESEM). Data were analyzed using paired t-test, and mixed ANOVA with post hoc Tukey’s HSD test.
Results: Ginger solution showed the highest reduction in dentin microhardness (p < 0.05) followed by EDTA, cat whiskers, clove, NaOCl and saline. Post hoc Tukey’s HSD test did not show any statistically significant difference in the reduction of the dentin microhardness between all irrigants (p > 0.05). Normal saline showed the most occluded dentinal tubules with the presence of superficial debris, whilst the remaining groups showed few occluded dentinal tubules with superficial debris.
Conclusions: Within the limitations of this study all the experimental plant-derived root canal irrigants did not significantly alter dentin microhardness and had similar effectiveness to remove the smear layer as conventional irrigants.
Methods: Eighty-four extracted permanent single-rooted premolars were selected. Microhardness analysis was conducted using Vickers indenter on sixty decoronated roots. Root canal preparation was carried out prior to longitudinal resection of the root specimens and immersion for 5 minutes in six experimental and control solutions (n=10), namely: Group 1: 0.5 % ginger, Group 2: 2.5 % cat whiskers, Group 3: 0.05 % clove, Group 4: 2.5 % sodium hypochlorite (NaOCl), Group 5: 17 % ethylenediaminetetraacetic acid (EDTA) and Group 6: 0.9 % normal saline. Microhardness values were determined before and after immersion. Smear layer removal (n=24) at the coronal middle and apical root third was analysed using field-emission scanning electron microscopy analysis (FESEM). Data were analyzed using paired t-test, and mixed ANOVA with post hoc Tukey’s HSD test.
Results: Ginger solution showed the highest reduction in dentin microhardness (p < 0.05) followed by EDTA, cat whiskers, clove, NaOCl and saline. Post hoc Tukey’s HSD test did not show any statistically significant difference in the reduction of the dentin microhardness between all irrigants (p > 0.05). Normal saline showed the most occluded dentinal tubules with the presence of superficial debris, whilst the remaining groups showed few occluded dentinal tubules with superficial debris.
Conclusions: Within the limitations of this study all the experimental plant-derived root canal irrigants did not significantly alter dentin microhardness and had similar effectiveness to remove the smear layer as conventional irrigants.