IADR Abstract Archives
ZNF582 Methylation and Oral Microbiota in Oral Cancer
Objectives: DNA methylation plays a crucial role in oral cancer. Specifically, methylation status of the ZNF582 gene is a significant prognostic factor for disease progression. Dysbiosis of the oral microbiota has also been implicated in carcinogenesis. However, the relationship between ZNF582 methylation and oral microbiota in oral cancer remains unclear. This study aimed to explore the associations between oral microbiota composition, ZNF582 methylation levels, and oral squamous cell carcinoma (OSCC) progression.
Methods: Mucosal samples were collected and subjected to Bisulfite conversion to assess DNA methylation, while next-generation sequencing (NGS) was employed to examine the composition of the oral microbiota.
Results: Participants were grouped based on methylation levels and pathological conditions. Analysis revealed that the OSCC group (n=14) exhibited higher bacteria species abundance compared to the healthy group (n=11), as determined by alpha diversity (Chao1, Shannon index), Beta diversity (PCA), and taxonomic composition analyses. Integrating ZNF582 DNA methylation with pathological characteristics showed significant differences in bacteria species diversity and abundance between OSCC patients and healthy individuals.
Conclusions: Results of this reveals a significant association between ZNF582 methylation, oral microbiota composition, and OSCC progression, emphasizing their potential as crucial pathophysiologic factors. These results contribute to the feasibility of early prediction of OSCC by identifying alterations in the oral microbiota during the carcinogenic process.
Methods: Mucosal samples were collected and subjected to Bisulfite conversion to assess DNA methylation, while next-generation sequencing (NGS) was employed to examine the composition of the oral microbiota.
Results: Participants were grouped based on methylation levels and pathological conditions. Analysis revealed that the OSCC group (n=14) exhibited higher bacteria species abundance compared to the healthy group (n=11), as determined by alpha diversity (Chao1, Shannon index), Beta diversity (PCA), and taxonomic composition analyses. Integrating ZNF582 DNA methylation with pathological characteristics showed significant differences in bacteria species diversity and abundance between OSCC patients and healthy individuals.
Conclusions: Results of this reveals a significant association between ZNF582 methylation, oral microbiota composition, and OSCC progression, emphasizing their potential as crucial pathophysiologic factors. These results contribute to the feasibility of early prediction of OSCC by identifying alterations in the oral microbiota during the carcinogenic process.