IADR Abstract Archives
Magnesium Enhances Osteogenic Differentiation of Human Periodontal Ligament Stem Cells
Objectives: Magnesium-based capping materials have potential in regenerative dentistry for promoting osteo/odontogenic differentiation of dental pulp stem cells. However, the impact of magnesium on human periodontal ligament stem cells (hPDLSCs), which could play a role in alveolar bone regeneration, remains elusive. This study aims to investigate the effects of magnesium on the proliferation and osteogenic differentiation of hPDLSCs.
Methods: hPDLSCs were isolated, characterized and cultured with magnesium chloride (MgCl2) at various concentrations (0.1-10 mM). Cell viability andproliferation were assessed using MTT assay. Colony-forming unit (CFU) and cell cycle analysis were examined by crystal violet and propidium iodide staining, respectively. Cell migration was measured by a scratch wound assay. Osteogenic differentiation and mineralization were assessed by alkaline phosphatase (ALP) activity, Alizarin Red S staining, and osteogenic-related gene expression using RT-qPCR. All statistical analyses were evaluated at p < 0.05.
Results: hPDLSCs showed the mesenchymal stem cell characteristics. MgCl2 concentrations higher than 10 mM exhibited cytotoxicity. Significant increase in cell proliferation rate, higher CFU percentages, and active cell cycle activity were observed when hPDLSCs were treated with 0.1, 0.5, and 1 mM MgCl2. However, MgCl2 had no effect on cell migration. Mineralized nodules were significantly observed in hPDLSCs treated with 0.1 and 0.5 mM MgCl2 in an osteogenic induction medium for 14 days. Conversely, hPDLSCs treated with 1, 5, and 10 mM MgCl2 exhibited lower levels of mineralization. Furthermore, hPDLSCs treated with 0.1 mM MgCl2 resulted in an increase in osteogenic marker gene expression, including RUNX2 and ALP.
Conclusions: MgCl2 at a concentration of 0.1 mM is the most effective dose to promote cell proliferation and stimulate osteogenic differentiation of hPDLSCs, in vitro. This data suggests the potential role of magnesium as a supplemental chemical in regenerative dentistry, particularly in alveolar bone regeneration
Methods: hPDLSCs were isolated, characterized and cultured with magnesium chloride (MgCl2) at various concentrations (0.1-10 mM). Cell viability andproliferation were assessed using MTT assay. Colony-forming unit (CFU) and cell cycle analysis were examined by crystal violet and propidium iodide staining, respectively. Cell migration was measured by a scratch wound assay. Osteogenic differentiation and mineralization were assessed by alkaline phosphatase (ALP) activity, Alizarin Red S staining, and osteogenic-related gene expression using RT-qPCR. All statistical analyses were evaluated at p < 0.05.
Results: hPDLSCs showed the mesenchymal stem cell characteristics. MgCl2 concentrations higher than 10 mM exhibited cytotoxicity. Significant increase in cell proliferation rate, higher CFU percentages, and active cell cycle activity were observed when hPDLSCs were treated with 0.1, 0.5, and 1 mM MgCl2. However, MgCl2 had no effect on cell migration. Mineralized nodules were significantly observed in hPDLSCs treated with 0.1 and 0.5 mM MgCl2 in an osteogenic induction medium for 14 days. Conversely, hPDLSCs treated with 1, 5, and 10 mM MgCl2 exhibited lower levels of mineralization. Furthermore, hPDLSCs treated with 0.1 mM MgCl2 resulted in an increase in osteogenic marker gene expression, including RUNX2 and ALP.
Conclusions: MgCl2 at a concentration of 0.1 mM is the most effective dose to promote cell proliferation and stimulate osteogenic differentiation of hPDLSCs, in vitro. This data suggests the potential role of magnesium as a supplemental chemical in regenerative dentistry, particularly in alveolar bone regeneration