IADR Abstract Archives
Distinctive Tooth Phenotype Associated with Osteogenesis Imperfecta
Objectives: This study aims to investigate and compare the properties of teeth in patients with osteogenesis imperfecta (OI) with and without dentinogenesis imperfecta (DI), in order to identify distinctive tooth phenotypic features associated with the conditions.
Methods: Primary teeth were collected from two individuals clinically diagnosed with OI type III, one with DI (OIDI) and the other without DI (OI). Tooth characterization was carried out, including the analysis of tooth color, surface roughness, hardness and elasticity, mineral density, and mineral content using a digital intraoral colorimeter, surface profilometer, micro-computerized tomography (Micro-CT), nano-base indentation system, and Energy-Dispersive X-ray (EDX), respectively. Furthermore, the ultrastructure of the teeth were observed using Scanning Electron Microscopy (SEM) and histology.
Results: OIDI teeth exhibited darker, yellower, and redder compared to OI teeth. Surface analysis demonstrated that OIDI teeth were smoother than both OI and control teeth. Micro-CT analysis revealed higher enamel density in OIDI teeth compared to OI and control teeth. Furthermore, nanohardness and elasticity of the enamel and dentin of OIDI teeth were significantly reduced compared to OI teeth. The calcium/phosphorus (Ca/P) ratio in the enamel and dentin of OIDI teeth was comparable to that of OI and control teeth. Ultrastructural examination of OIDI teeth revealed a marked reduction in dentinal tubule number, as well as the presence of spare, obstructed, and disorganized dentinal tubules. In contrast, the ultrastructure of OI teeth were similar to those of control teeth.
Conclusions: This study provides insight into the distinct tooth characteristics observed in OI patients with DI. The reduced hardness and elasticity, along with the malformation of dentin structure observed in OIDI teeth, could contribute to their increased brittleness and potential failure of adhesive restorations. These findings expand our understanding of the phenotypic spectrum in OI patients, both with and without DI, and emphasize the importance of raising awareness among dental practitioners regarding the unique management considerations for individuals with OI.
Methods: Primary teeth were collected from two individuals clinically diagnosed with OI type III, one with DI (OIDI) and the other without DI (OI). Tooth characterization was carried out, including the analysis of tooth color, surface roughness, hardness and elasticity, mineral density, and mineral content using a digital intraoral colorimeter, surface profilometer, micro-computerized tomography (Micro-CT), nano-base indentation system, and Energy-Dispersive X-ray (EDX), respectively. Furthermore, the ultrastructure of the teeth were observed using Scanning Electron Microscopy (SEM) and histology.
Results: OIDI teeth exhibited darker, yellower, and redder compared to OI teeth. Surface analysis demonstrated that OIDI teeth were smoother than both OI and control teeth. Micro-CT analysis revealed higher enamel density in OIDI teeth compared to OI and control teeth. Furthermore, nanohardness and elasticity of the enamel and dentin of OIDI teeth were significantly reduced compared to OI teeth. The calcium/phosphorus (Ca/P) ratio in the enamel and dentin of OIDI teeth was comparable to that of OI and control teeth. Ultrastructural examination of OIDI teeth revealed a marked reduction in dentinal tubule number, as well as the presence of spare, obstructed, and disorganized dentinal tubules. In contrast, the ultrastructure of OI teeth were similar to those of control teeth.
Conclusions: This study provides insight into the distinct tooth characteristics observed in OI patients with DI. The reduced hardness and elasticity, along with the malformation of dentin structure observed in OIDI teeth, could contribute to their increased brittleness and potential failure of adhesive restorations. These findings expand our understanding of the phenotypic spectrum in OI patients, both with and without DI, and emphasize the importance of raising awareness among dental practitioners regarding the unique management considerations for individuals with OI.