IADR Abstract Archives
Cellular responses of biogenic source-derived scaffolds prepared from fish bones
Objectives: This study aims to investigate the biogenic source-derived scaffolds (BS) fabricated from Nile Tilapia bone (FB) on morphology, cytotoxicity, and migration of human periodontal ligament cells (hPDLCs).
Methods: The BS-FB was fabricated from Nile tilapia (Oreochromis niloticus) bones using thermal decomposition methods, with three different calcination temperatures (500, 600, and 700 °C). The attachment and morphology of hPDLCs cultured on the BS-FB were observed using a scanning electron microscope. Cell viability and proliferation were determined using the MTT assay according to ISO 10993-5:2009 at 1, 3, and 7 days. Cell migration was examined using a wound healing assay at 1, 3, and 7 days. The cell cultured without BS-FB were served as the positive control. The data were analyzed using one-way ANOVA followed by Tukey's post-hoc analysis, with a significance level of 0.05.
Results: hPDLCs cultured for 1, 3, and 7 days exhibited a spindle-shaped appearance and demonstrated good adhesion on the scaffold surface in all groups. According to the MTT assay, cell viability was significantly higher in BS-FB500, BS-FB600, and BS-FB700 compared to the positive control after 3 and 7 days (p<0.001). The wound healing assay revealed a significantly higher percentage of cell migration in BS-FB500, BS-FB600, and BS-FB700 compared to the positive control after 1, 3, and 7 days (p<0.001). Furthermore, BS-FB600 exhibited the highest percentage of cell migration compared to the other groups after 1, 3, and 7 days (p<0.05).
Conclusions: This study demonstrated that the BS-FB exhibits cellular biocompatibility with hPDLCs. Further studies should be conducted to investigate its physical, chemical, and mechanical properties, as well as its effects on the induced osteogenic differentiation of hPDLCs. Biogenic source-derived scaffolds fabricated from Nile Tilapia bones present a promising alternative for orthopedic biomedical applications and bone regeneration in the future.
Methods: The BS-FB was fabricated from Nile tilapia (Oreochromis niloticus) bones using thermal decomposition methods, with three different calcination temperatures (500, 600, and 700 °C). The attachment and morphology of hPDLCs cultured on the BS-FB were observed using a scanning electron microscope. Cell viability and proliferation were determined using the MTT assay according to ISO 10993-5:2009 at 1, 3, and 7 days. Cell migration was examined using a wound healing assay at 1, 3, and 7 days. The cell cultured without BS-FB were served as the positive control. The data were analyzed using one-way ANOVA followed by Tukey's post-hoc analysis, with a significance level of 0.05.
Results: hPDLCs cultured for 1, 3, and 7 days exhibited a spindle-shaped appearance and demonstrated good adhesion on the scaffold surface in all groups. According to the MTT assay, cell viability was significantly higher in BS-FB500, BS-FB600, and BS-FB700 compared to the positive control after 3 and 7 days (p<0.001). The wound healing assay revealed a significantly higher percentage of cell migration in BS-FB500, BS-FB600, and BS-FB700 compared to the positive control after 1, 3, and 7 days (p<0.001). Furthermore, BS-FB600 exhibited the highest percentage of cell migration compared to the other groups after 1, 3, and 7 days (p<0.05).
Conclusions: This study demonstrated that the BS-FB exhibits cellular biocompatibility with hPDLCs. Further studies should be conducted to investigate its physical, chemical, and mechanical properties, as well as its effects on the induced osteogenic differentiation of hPDLCs. Biogenic source-derived scaffolds fabricated from Nile Tilapia bones present a promising alternative for orthopedic biomedical applications and bone regeneration in the future.