IADR Abstract Archives
Role of ECM-derived from ICF-treated PDL cells on osteogenic differentiation
Objectives: PDLs are considered as mechanosensory cells in responding to force. ICF is mechanical stimulation related to mastication in the regulation of homeostasis of hPDLs activity. This study aims to investigate the effect of extracellular matrix (ECM) expression (ECM)-derived from intermittent compressive force (ICF)-treated human periodontal ligament cells (PDLs) on osteogenic differentiation.
Methods: PDLs were seeded on 24 well-plate for 24h and loaded with ICF at 1.5g/cm2 for 24 h in serum-free medium and maintained with normal medium (N) and osteogenic induction medium (OM) followed by a decellularization process. Decellularized ECM (dECM) was characterized using SEM and EDX analysis. PDLs were reseeded on dECM and cell viability was examined by resazurin assay and LIVE/DEAD assay. Mineralization was determined by Alizarin Red S staining.
Results: ECM derived from ICF-treated PDLs exhibited fibrillar structure. ECM derived from ICF-treated PDLs cultured in OM demonstrated the upregulation of fibronectin and type I of collagen expression. dECM from both N and OM conditions did not exhibit cytotoxicity. Cells were able to attach and proliferate on dECM. The dECM derived from ICF-treated PDLs cultured in OM significantly enhanced mineral deposition compared to that dECM derived from PDLs in normal condition.
Conclusions: dECM derived from ICF-treated PDLs supports cell growth and differentiation. This could be further utilized as an alternative biomaterial for regenerative treatment.
Methods: PDLs were seeded on 24 well-plate for 24h and loaded with ICF at 1.5g/cm2 for 24 h in serum-free medium and maintained with normal medium (N) and osteogenic induction medium (OM) followed by a decellularization process. Decellularized ECM (dECM) was characterized using SEM and EDX analysis. PDLs were reseeded on dECM and cell viability was examined by resazurin assay and LIVE/DEAD assay. Mineralization was determined by Alizarin Red S staining.
Results: ECM derived from ICF-treated PDLs exhibited fibrillar structure. ECM derived from ICF-treated PDLs cultured in OM demonstrated the upregulation of fibronectin and type I of collagen expression. dECM from both N and OM conditions did not exhibit cytotoxicity. Cells were able to attach and proliferate on dECM. The dECM derived from ICF-treated PDLs cultured in OM significantly enhanced mineral deposition compared to that dECM derived from PDLs in normal condition.
Conclusions: dECM derived from ICF-treated PDLs supports cell growth and differentiation. This could be further utilized as an alternative biomaterial for regenerative treatment.