IADR Abstract Archives
Efficacy of Chitosan Intracanal Medication against Fungal-bacterial Dual-species Biofilm
Objectives: Successful endodontic treatment depends on the ability to control intracanal infection. Multispecies biofilm composing of both fungal and bacterial species are particularly difficult to eradicate. Candida albicans and Enterococcus faecalis are examples of common microorganisms in persistent root canal infection and their dual-species biofilms showed increased resistance to treatment. Thus, we aimed to investigate the efficacy of an intracanal chitosan paste on the dual-species biofilm in an in vitro root canal infection model.
Methods: Twenty-four root samples were prepared from extracted human premolars and incisors and were infected with C. albicans and E. faecalis at 1:1 ratio and incubated at 37°C for 3 days for dual-species biofilm formation. The samples were randomly assigned to 4 groups of intracanal medication for 7 days: 1. negative control, 2. calcium hydroxide (Ca(OH)2), 3. propylene glycol (PG) and 4. chitosan (1700 kD) + PG. After removal of the medication, intracanal dentin was collected using Protaper next, and the number of colony forming units (CFUs) were counted from surviving colonies on Brain-Heart-Infusion agar (for E. faecalis) and Yeast Extract-Peptone-Dextrose agar (for C. albicans). The percentage of remaining CFUs relative to negative control was calculated and analyzed using One-way ANOVA and post-hoc Games-Howell test. The significance level was set at 0.05.
Results: Our results showed that although calcium hydroxide and PG could reduce the number of viable microorganisms compared with negative control (P<0.001), chitosan+PG paste showed highest antimicrobial activity (P<0.001, 0.008, and 0.095 comparing to negative control, calcium hydroxide, and PG, respectively).
Conclusions: In conclusion, chitosan paste showed the highest efficacy against C.albicans and E. faecalis dual-species biofilm after 7-day medication in an in vitro root canal infection model.
Methods: Twenty-four root samples were prepared from extracted human premolars and incisors and were infected with C. albicans and E. faecalis at 1:1 ratio and incubated at 37°C for 3 days for dual-species biofilm formation. The samples were randomly assigned to 4 groups of intracanal medication for 7 days: 1. negative control, 2. calcium hydroxide (Ca(OH)2), 3. propylene glycol (PG) and 4. chitosan (1700 kD) + PG. After removal of the medication, intracanal dentin was collected using Protaper next, and the number of colony forming units (CFUs) were counted from surviving colonies on Brain-Heart-Infusion agar (for E. faecalis) and Yeast Extract-Peptone-Dextrose agar (for C. albicans). The percentage of remaining CFUs relative to negative control was calculated and analyzed using One-way ANOVA and post-hoc Games-Howell test. The significance level was set at 0.05.
Results: Our results showed that although calcium hydroxide and PG could reduce the number of viable microorganisms compared with negative control (P<0.001), chitosan+PG paste showed highest antimicrobial activity (P<0.001, 0.008, and 0.095 comparing to negative control, calcium hydroxide, and PG, respectively).
Conclusions: In conclusion, chitosan paste showed the highest efficacy against C.albicans and E. faecalis dual-species biofilm after 7-day medication in an in vitro root canal infection model.