IADR Abstract Archives
Antimicrobial susceptibility studies on a potentially haemostatic Camellia sinensis extract.
Objectives: Oral and maxillofacial infections have either an odontogenic or a non-odontogenic aetiology. The oral cavity is a reservoir of 774 bacterial species as documented in the expanded Human Oral Microbiome Database (eHOMD) v3.1. Staphylococcus is an opportunistic non-fastidious gram-positive bacterium, a commensal of the oral cavity, nasal cavity, and cutaneous tissues in healthy homo sapiens. This in vitro study aimed to confirm the antimicrobial susceptibility of six Camellia sinensis variety assamica tea leaves extracts (a potential dental haemostatic agent) on the Staphylococcus aureus subspecies aureus Rosenbach ATCCÒ 25923™ strain.
Methods: Disk-diffusion tests (DDT) were conducted where Staphylococcus aureus (S.aureus) 25923™ inocula, incubated in tryptone soy broth (TSB) for a minimum of 20 hours were suspended on Mueller-Hinton agar plates in triplicate (CLSI supplement M100.33Ed.). Commercialised antibiotic discs[OXOID™] of AML10:amoxycillin (10 mg), AMC30:amoxycillin + clavulanate (30 mg), and CXM30:cefuroxime (30 mg) were placed as the positive control in three different sets of DDT, while 6.0mm filter discs of deionised water (10 mL) were placed as the negative control to exclude experimental bias in this study. 10 mL of six types of tea leave samples were placed on 6.0mm filter discs. CSALE samples comprised independently extracted fresh green tea leaves and commercialised black tea leaves from a single, locally sourced highland plantation, fresh green tea leaves: GTE1, GTE2, and GTE3 whereas commercialised black tea leaves: BTE1, BTE2, and BTE3.
Results: Our study shows that all Camellia sinensis variety assamica extracts have antimicrobial susceptibility, with GTE1 showing the highest susceptibility against Staphylococcus aureus 25923™, mean zone of inhibition in diameter: 21.3mm (AML 10); 21.0mm (AMC30), and 13.0mm (CXM30) when compared to the other five extracts.
Conclusions: In conclusion, GTE1, an extraction of fresh green tea leaves has shown high susceptibility ratio and bears no harm in the treatment of odontogenic or non-odontogenic oral and maxillofacial haemorrhage.
Methods: Disk-diffusion tests (DDT) were conducted where Staphylococcus aureus (S.aureus) 25923™ inocula, incubated in tryptone soy broth (TSB) for a minimum of 20 hours were suspended on Mueller-Hinton agar plates in triplicate (CLSI supplement M100.33Ed.). Commercialised antibiotic discs[OXOID™] of AML10:amoxycillin (10 mg), AMC30:amoxycillin + clavulanate (30 mg), and CXM30:cefuroxime (30 mg) were placed as the positive control in three different sets of DDT, while 6.0mm filter discs of deionised water (10 mL) were placed as the negative control to exclude experimental bias in this study. 10 mL of six types of tea leave samples were placed on 6.0mm filter discs. CSALE samples comprised independently extracted fresh green tea leaves and commercialised black tea leaves from a single, locally sourced highland plantation, fresh green tea leaves: GTE1, GTE2, and GTE3 whereas commercialised black tea leaves: BTE1, BTE2, and BTE3.
Results: Our study shows that all Camellia sinensis variety assamica extracts have antimicrobial susceptibility, with GTE1 showing the highest susceptibility against Staphylococcus aureus 25923™, mean zone of inhibition in diameter: 21.3mm (AML 10); 21.0mm (AMC30), and 13.0mm (CXM30) when compared to the other five extracts.
Conclusions: In conclusion, GTE1, an extraction of fresh green tea leaves has shown high susceptibility ratio and bears no harm in the treatment of odontogenic or non-odontogenic oral and maxillofacial haemorrhage.