IADR Abstract Archives
Oral Veillonella Profiles Associated With Stunted-Groups in Indonesian Children’s Dental-Biofilm
Objectives: Stunting is one of the most burdening health problems in Indonesia, especially in East Nusa Tenggara Province, with a prevalence of 41.1% in children between 5-12 years old. Additionally, a higher proportion of Veillonella was reported in children with poor oral hygiene. However, the relationship between oral populations of Veillonella and stunting has not been studied before. Therefore, this study aimed to analyze the oral Veillonella profile from dental biofilms of healthy and stunted children aged 6–7 years.
Methods: 60 school children (Table1, boys=26, girls=34) between the age of 6-7 were participate in this study. The subjects were then divided into healthy group (n=31, HAZ≥-2.0) and stunted group (n=29, HAZ<-2.0) based on WHO definition of stunting. 4 calibrated dentists then evaluated and classified the subjects into good oral hygiene (0-1.2), moderate oral hygiene (1.3-3.0), and poor oral hygiene (3.1-6.0) based on the simplified oral hygiene index (OHI-S). Dental-biofilm samples were extracted and diluted in 1 mL of phosphate-buffer saline and stored in -20°C storage. The DNA from the samples were then extracted for further absolute quantification by qPCR. Primers for 7 oral Veillonella species were used for each oral Veillonella species. Oral Veillonella species standard curve were made using ATCC (R2>0,985) to quantify each species. Statistical analysis were then performed using SPSS.
Results: All oral Veillonella species were detected in all samples. The statistical analysis revealed four oral Veillonella species significantly differed between healthy and stunted groups such as V. denticariosi, V. infantium, V. rogosae and V. tobetsuensis (Fig 1).
Conclusions: This is the first study shown the potential association between oral Veillonella species and stunting in children. Further studies are required to examine the properties of oral Veillonella species in order to provide the understanding of their role in stunting.
Methods: 60 school children (Table1, boys=26, girls=34) between the age of 6-7 were participate in this study. The subjects were then divided into healthy group (n=31, HAZ≥-2.0) and stunted group (n=29, HAZ<-2.0) based on WHO definition of stunting. 4 calibrated dentists then evaluated and classified the subjects into good oral hygiene (0-1.2), moderate oral hygiene (1.3-3.0), and poor oral hygiene (3.1-6.0) based on the simplified oral hygiene index (OHI-S). Dental-biofilm samples were extracted and diluted in 1 mL of phosphate-buffer saline and stored in -20°C storage. The DNA from the samples were then extracted for further absolute quantification by qPCR. Primers for 7 oral Veillonella species were used for each oral Veillonella species. Oral Veillonella species standard curve were made using ATCC (R2>0,985) to quantify each species. Statistical analysis were then performed using SPSS.
Results: All oral Veillonella species were detected in all samples. The statistical analysis revealed four oral Veillonella species significantly differed between healthy and stunted groups such as V. denticariosi, V. infantium, V. rogosae and V. tobetsuensis (Fig 1).
Conclusions: This is the first study shown the potential association between oral Veillonella species and stunting in children. Further studies are required to examine the properties of oral Veillonella species in order to provide the understanding of their role in stunting.
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