IADR Abstract Archives
Membrane Vesicles Improve Streptococcus Mutans Biofilm Formation
Objectives: One of the main etiological pathogen of dental caries is Streptococcus mutans (S. mutans), which can form biofilm known as dental plaque and cause dental decay. Although there is increasing information about the effects of bacteria membrane vesicles (MVs) on biofilm, few data are available on MVs generated by S. mutans. The objective of this study is to assess the effect of MVs derived from S. mutans on biofilm formation of S. mutans.
Methods: Transmission electron microscopy and nanoparticle tracking analysis were used to observe the shape of MVs. The effect of adding MVs in S. mutans biofilm was detected by crystal violet stain, XTT assay, confocal laser scanning microscope and scanning electron microscopy. Real time PCR was used to detect the expression of the related virulence factors of S. mutans in the biofilm treated with MVs.
Results: Transmission electron microscopy showed that the morphology of MVs was cup shaped with bilayered membranes. Nanoparticle tracking analysis showed MVs was 80.49±32.24 nm diameter. Based on crystal violet assay and XTT assay, more than 5 μg/ml supplementary MVs could improve biofilm formation at the initial adhesion stage (0 to 6 hours). Confocal laser scanning microscope and scanning electron microscopy verified that PKH26 labeled MVs could be seen in S. mutans biofilm and the supplementary MVs would improve the quantity of live bacteria and the biomass of extracellular polysaccharide. Moreover, the expression of genes related to adhesion and quorum-sensing systems was increased after MVs supplementary.
Conclusions: In conclusion, S. muants MVs act as a component of S. mutans biofilms, which can increase the biofilm information at the early stage of biofilm formation.
Methods: Transmission electron microscopy and nanoparticle tracking analysis were used to observe the shape of MVs. The effect of adding MVs in S. mutans biofilm was detected by crystal violet stain, XTT assay, confocal laser scanning microscope and scanning electron microscopy. Real time PCR was used to detect the expression of the related virulence factors of S. mutans in the biofilm treated with MVs.
Results: Transmission electron microscopy showed that the morphology of MVs was cup shaped with bilayered membranes. Nanoparticle tracking analysis showed MVs was 80.49±32.24 nm diameter. Based on crystal violet assay and XTT assay, more than 5 μg/ml supplementary MVs could improve biofilm formation at the initial adhesion stage (0 to 6 hours). Confocal laser scanning microscope and scanning electron microscopy verified that PKH26 labeled MVs could be seen in S. mutans biofilm and the supplementary MVs would improve the quantity of live bacteria and the biomass of extracellular polysaccharide. Moreover, the expression of genes related to adhesion and quorum-sensing systems was increased after MVs supplementary.
Conclusions: In conclusion, S. muants MVs act as a component of S. mutans biofilms, which can increase the biofilm information at the early stage of biofilm formation.