IADR Abstract Archives
Inhibitory Activity of Stachytarpheta Jamaicensis (L.) Vahl Roots Extract on Some Selected Mouth Pathogenic Bacteria
Objectives: To determine the in vitro inhibitory activity of S. jamaicensis roots extract (SJRE) on some mouth pathogenic bacteria
Methods: Each of Aggregatibacter actinomycetemcomitans, Actinomyces sp., Enterococcus faecalis were cultured at the Laboratory of Department of Biomedical. S. jamaicensis roots extract was prepared by 96% ethanol extraction method and diluted from 50 to 1.56 mg/ml. Standard techniques broth microdilution assays were applied for quantitative determinations of antibacterial activity of S. jamaicensis extract on each isolate.
Results: All bacteria were sensitive to SJRE at concentrations 125µg/ml, 250µg/ml, 500µg/ml, 1000µg/ml, 2000µg/ml, 4000µg/ml, 8000µg/ml producing inhibition growth of bacteria. All bacteria tested were sensitive to 2000µg/ml, 4000µg/ml, 8000µg/ml of this extract. The minimal inhibitory concentration (MIC) of SJRE extract for A. actinomycetemcomitans was 2000 µg/m, for Actinomyces sp. was 8000µg/m, and for Enterococcus faecalis was 2000µg/m, but there was no minimum bactericidal concentration (MBC).
Conclusions: Our findings showed that SJRE exhibited antibacterial activity on A. actinomycetemcomitans, Actinomyces sp., Enterococcus faecalis and therefore may be used in mouthwashes or dentifrices for prevention of dental caries and periodontal diseases.
Methods: Each of Aggregatibacter actinomycetemcomitans, Actinomyces sp., Enterococcus faecalis were cultured at the Laboratory of Department of Biomedical. S. jamaicensis roots extract was prepared by 96% ethanol extraction method and diluted from 50 to 1.56 mg/ml. Standard techniques broth microdilution assays were applied for quantitative determinations of antibacterial activity of S. jamaicensis extract on each isolate.
Results: All bacteria were sensitive to SJRE at concentrations 125µg/ml, 250µg/ml, 500µg/ml, 1000µg/ml, 2000µg/ml, 4000µg/ml, 8000µg/ml producing inhibition growth of bacteria. All bacteria tested were sensitive to 2000µg/ml, 4000µg/ml, 8000µg/ml of this extract. The minimal inhibitory concentration (MIC) of SJRE extract for A. actinomycetemcomitans was 2000 µg/m, for Actinomyces sp. was 8000µg/m, and for Enterococcus faecalis was 2000µg/m, but there was no minimum bactericidal concentration (MBC).
Conclusions: Our findings showed that SJRE exhibited antibacterial activity on A. actinomycetemcomitans, Actinomyces sp., Enterococcus faecalis and therefore may be used in mouthwashes or dentifrices for prevention of dental caries and periodontal diseases.