IADR Abstract Archives
Whole-Transcriptiome Sequencing Identifies the Expression Profile of LncRNAs, CircRNAs, MiRNAs, and MRNAs in the Trigeminal Ganglion Associated With Trigeminal Neuropathic Pain
Objectives: Trigeminal neuropathic pain is a significant health problem. Recent studies have proved non-coding RNAs (ncRNAs) contribute to the development of neuropathic pain. However, it is still unclear that the expression profile of ncRNAs in the trigeminal ganglion (TG) and their functional mechanisms in trigeminal neuropathic pain. This study was aimed to identify the expression profile of long noncoding RNAs (lncRNA), circular RNAs (circRNAs), micro RNAs (miRNA) and message RNAs (mRNA) in the TG and their regulatory function in trigeminal neuropathic pain.
Methods: Chronic constriction injury of the infraorbital nerve (CCI-ION) of mouse was performed to establish trigeminal neuropathic pain model. Von Frey filament test was performed to examine the head withdrawal threshold (HWT) of mice. RNA-seq was used to identify the expression profile of ncRNAs and mRNAs in the TG. RT-qPCR was performed to prove the expression of selected ncRNAs and mRNAs. The potential biological functions of differentially expressed (DE) ncRNAs and DE mRNAs were analyzed by bioinformatics analysis.
Results: The HWT of CCI-ION mice significantly decreased since from 3 days after surgery. Totally, 216 lncRNAs, 14 circRNAs, 67 miRNAs, and 595 mRNAs were differentally expressed in the TG on 7 days after CCI-ION surgery. The results showed that 39 DEGs were known pain genes. GO analysis indicated that response to stress,membrane related cell components, binding related molecular functions were enriched. KEGG analysis showed that the most significantly involved pathways were ECM-receptor interaction, protein digestion and absorption, PI3K-Akt signaling pathway. Besides, the lncRNA/circRNA-miRNA-mRNA regulatory networks based on the ceRNA regulatory principle existed under the condition of trigeminal neuropathic pain.
Conclusions: The findings in this study demonstrate ncRNAs play an important role in the development of trigeminal neuropathic pain, which indicates novel mechanisms underlying trigeminal neuropathic pain and bright novel therapeutic targets for orofacial pain targeting ncRNAs.
Methods: Chronic constriction injury of the infraorbital nerve (CCI-ION) of mouse was performed to establish trigeminal neuropathic pain model. Von Frey filament test was performed to examine the head withdrawal threshold (HWT) of mice. RNA-seq was used to identify the expression profile of ncRNAs and mRNAs in the TG. RT-qPCR was performed to prove the expression of selected ncRNAs and mRNAs. The potential biological functions of differentially expressed (DE) ncRNAs and DE mRNAs were analyzed by bioinformatics analysis.
Results: The HWT of CCI-ION mice significantly decreased since from 3 days after surgery. Totally, 216 lncRNAs, 14 circRNAs, 67 miRNAs, and 595 mRNAs were differentally expressed in the TG on 7 days after CCI-ION surgery. The results showed that 39 DEGs were known pain genes. GO analysis indicated that response to stress,membrane related cell components, binding related molecular functions were enriched. KEGG analysis showed that the most significantly involved pathways were ECM-receptor interaction, protein digestion and absorption, PI3K-Akt signaling pathway. Besides, the lncRNA/circRNA-miRNA-mRNA regulatory networks based on the ceRNA regulatory principle existed under the condition of trigeminal neuropathic pain.
Conclusions: The findings in this study demonstrate ncRNAs play an important role in the development of trigeminal neuropathic pain, which indicates novel mechanisms underlying trigeminal neuropathic pain and bright novel therapeutic targets for orofacial pain targeting ncRNAs.