IADR Abstract Archives
Tideglusib Enhances Odontogenic Differentiation in Human Dental Pulp Stem Cells
Objectives: Tideglusib is a small molecule agonist of the canonical Wnt pathway. The present study investigated the influence of Tideglusib on human dental pulp stem cells (hDPSCs) proliferation, apoptosis, migration, and odonto/osteogenic differentiation
Methods: hDPSCs were treated with 50 nM, 100 nM, or 200 nM Tideglusib. β-catenin accumulation was detected by immunofluorescence staining. Colony forming unit ability was assessed by staining with Coomassie blue. Cell cycle progression and cell apoptosis were investigated using flow cytometry. Cell migration was examined using an in vitro wound healing assay. Osteogenic differentiation was examined using alkaline phosphatase staining, alizarin red staining, and osteogenic-related gene expression. Gene expression profile was examined using a high throughput RNA sequencing technique
Results: Tideglusib activated the Wnt signaling pathway in hDPSCs as demonstrated by an increase in cytoplasmic β-catenin accumulation and nuclear translocation. Tideglusib did not affect hDPSCs proliferation, cell cycle progression, cell apoptosis, or cell migration. In contrast, 50 nM and 100 nM Tideglusib significantly enhanced mineralization and osteogenic marker gene expression (RUNX2, ALP, BMP2, and DSPP)
Conclusions: Tideglusib enhanced the odonto/osteogenic differentiation of hDPSCs. Further, incorporating this bioactive molecule in a pulp capping material could be a promising strategy to promote dentin repair
Methods: hDPSCs were treated with 50 nM, 100 nM, or 200 nM Tideglusib. β-catenin accumulation was detected by immunofluorescence staining. Colony forming unit ability was assessed by staining with Coomassie blue. Cell cycle progression and cell apoptosis were investigated using flow cytometry. Cell migration was examined using an in vitro wound healing assay. Osteogenic differentiation was examined using alkaline phosphatase staining, alizarin red staining, and osteogenic-related gene expression. Gene expression profile was examined using a high throughput RNA sequencing technique
Results: Tideglusib activated the Wnt signaling pathway in hDPSCs as demonstrated by an increase in cytoplasmic β-catenin accumulation and nuclear translocation. Tideglusib did not affect hDPSCs proliferation, cell cycle progression, cell apoptosis, or cell migration. In contrast, 50 nM and 100 nM Tideglusib significantly enhanced mineralization and osteogenic marker gene expression (RUNX2, ALP, BMP2, and DSPP)
Conclusions: Tideglusib enhanced the odonto/osteogenic differentiation of hDPSCs. Further, incorporating this bioactive molecule in a pulp capping material could be a promising strategy to promote dentin repair