IADR Abstract Archives
Coffee Bean Extract Inhibits Oxidative Stress Due to Nickel and Cobalt Exposures in Human Peripheral Blood Mononuclear Cells
Objectives: To investigate potential effect of coffee bean extract against radical superoxide production in PBMC cells exposed to Ni and Co
Methods: The metal included NiCl2.6H2O and CoCl2.6H2O. The coffee extract is taken from East Java robusta coffee beans, they were macerated in 97% ethanol subsequently diluted into three concentrations: 125, 62.5, and 31.25 μg/ml. Oxidative stress activity was monitored by assessment of release of ROS production by using NBT assay
Results: The robusta coffee bean extract concentration of 125 μg/ml showed the optimal concentration in reducing radical superoxide in PBMC cells-induced by Ni and Co followed by exposure to 62.5 and 31.25 μg/ml.
Conclusions: Data obtained in the present study reveal that coffee bean extracts have capacity to inhibit radical superoxide due to Ni and Co exposures, which display potent PBMC oxidative activity capacities. More robust and well-controlled studies are still needed for a thorough understanding of the effect of coffee on other inflammatory markers in humans.
Methods: The metal included NiCl2.6H2O and CoCl2.6H2O. The coffee extract is taken from East Java robusta coffee beans, they were macerated in 97% ethanol subsequently diluted into three concentrations: 125, 62.5, and 31.25 μg/ml. Oxidative stress activity was monitored by assessment of release of ROS production by using NBT assay
Results: The robusta coffee bean extract concentration of 125 μg/ml showed the optimal concentration in reducing radical superoxide in PBMC cells-induced by Ni and Co followed by exposure to 62.5 and 31.25 μg/ml.
Conclusions: Data obtained in the present study reveal that coffee bean extracts have capacity to inhibit radical superoxide due to Ni and Co exposures, which display potent PBMC oxidative activity capacities. More robust and well-controlled studies are still needed for a thorough understanding of the effect of coffee on other inflammatory markers in humans.