IADR Abstract Archives
Antibacterial Efficacy of Contemporary Bioactive Dental Restoratives Against Streptococcus Mutans
Objectives: Bioactive glass dental restoratives have been introduced to overcome the challenge of secondary caries development in conventional composite-resin and glass ionomer restorations. However, there is limited evidence of the antibacterial efficacy of these contemporary bioactive restoratives. Therefore, the objective of this study was to compare the antibacterial efficacy of contemporary bioactive restoratives and conventional restoratives against S. mutans.
Methods: Conventional composite-resin (CR) and high viscosity glass ionomer (GI) as well as three contemporary bioactive restoratives, Giomer (GM), zirconia reinforced glass ionomer (ZC), and alkasite material (AK), were selected. Samples (n=6) were prepared using a stainless-steel mold, immersed in distilled water (37°C, 7d) and sterilized. Crystal violet assay and colony forming units (CFU) count were performed after samples were exposed to S. mutans suspension (24h) to determine bacterial biomass and S. mutans viability respectively. Surface roughness was measured for each sample. Results were statistically analyzed (Kruskal-Wallis, p<0.05). Correlation between surface roughness and S. mutans viability was determined (Spearman’s rank correlation coefficient, p<0.05).
Results: Our study reported the lowest median bacterial biomass (OD570nm) for CR (0.19), followed by GM (0.25), AK (0.47), GI (0.74) and ZC (0.89). However, we reported the lowest S. mutans viability (LogCFU/ml) for GM (2.13), followed by CR (2.38), AK (2.84), GI (2.98), and ZC (3.53). The lowest surface roughness was measured for CR, followed by GM, AK, GI and ZC. All groups had statistically significant differences in bacterial biomass (P<0.001), S. mutans viability (P<0.001) and surface roughness (P<0.001). A strong positive correlation coefficient of 0.874 was detected between surface roughness and S. mutans viability.
Conclusions: Resin-based groups displayed stronger antibacterial efficacy against S. mutans compared to GI-based groups. Our study suggests that surface roughness may play an important role in antibacterial efficacy; hence clinicians should consider this parameter in their choice of restorative materials.
Methods: Conventional composite-resin (CR) and high viscosity glass ionomer (GI) as well as three contemporary bioactive restoratives, Giomer (GM), zirconia reinforced glass ionomer (ZC), and alkasite material (AK), were selected. Samples (n=6) were prepared using a stainless-steel mold, immersed in distilled water (37°C, 7d) and sterilized. Crystal violet assay and colony forming units (CFU) count were performed after samples were exposed to S. mutans suspension (24h) to determine bacterial biomass and S. mutans viability respectively. Surface roughness was measured for each sample. Results were statistically analyzed (Kruskal-Wallis, p<0.05). Correlation between surface roughness and S. mutans viability was determined (Spearman’s rank correlation coefficient, p<0.05).
Results: Our study reported the lowest median bacterial biomass (OD570nm) for CR (0.19), followed by GM (0.25), AK (0.47), GI (0.74) and ZC (0.89). However, we reported the lowest S. mutans viability (LogCFU/ml) for GM (2.13), followed by CR (2.38), AK (2.84), GI (2.98), and ZC (3.53). The lowest surface roughness was measured for CR, followed by GM, AK, GI and ZC. All groups had statistically significant differences in bacterial biomass (P<0.001), S. mutans viability (P<0.001) and surface roughness (P<0.001). A strong positive correlation coefficient of 0.874 was detected between surface roughness and S. mutans viability.
Conclusions: Resin-based groups displayed stronger antibacterial efficacy against S. mutans compared to GI-based groups. Our study suggests that surface roughness may play an important role in antibacterial efficacy; hence clinicians should consider this parameter in their choice of restorative materials.