IADR Abstract Archives
Reuterin Inhibits Porphyromonas Gingivalis Virulence Genes in Root Canal Biofilm Model
Objectives: The compound reuterin isolated from the probiotic Lactobacillus reuteri is known to exhibit antimicrobial activity against a wide range of organisms, including oral pathogens. The aim of this study was to determine the effect of reuterin against fimA, mfa1, hagB genes expression in Porphyromonas gingivalis biofilm in an ex vivo root canal model.
Methods: The minimum inhibitory concentration (MIC) of reuterin for P. gingivalis ATCC 33277 were determined using MIC assays in 96-well plates. A total of 24 human premolar root canal samples had been prepared and sterilized. All dental root samples were inoculated with P.gingivalis bacteria that had been cultured for 24 hours at 37oC. Samples were divided into 4 groups according to irrigant types, group 1 were reuterin isolated from L. reuteri LC 382415 (Indonesian strain) 12,5 ug/ml, group 2 were reuterin isolated from L. reuteri Prodentis (BioGaia, Stockholm, Sweden), group 3 were NaOCl 2,5 % (positive control), group 4 were saline (negative control). Each irrigant types were divided into 2 contact times, 5 minutes and 30 minutes. Subsequently, genes expression quantification were determined by RT-PCR. Data were analyzed using one-way ANOVA with P<0.05 as level of significance.
Results: MIC of reuterin for P. gingivalis were found to be 12.5 μg/mL. Statistically, reuterin 12.5 μg/mL significantly reduced the transcription of fimA, mfa1, and hagB gene expression to form P. gingivalis biofilm in an ex vivo root canal model both at 5 minutes and 30 minutes contact time (P<0.05)
Conclusions: The results indicated that the anti-biofilm activity of reuterin against P. gingivalis may be due to attenuating the virulence genes. Hence, reuterin may develop as a potential source for future endodontic treatment. However, further clinical research is required for concerete conclusions.
Methods: The minimum inhibitory concentration (MIC) of reuterin for P. gingivalis ATCC 33277 were determined using MIC assays in 96-well plates. A total of 24 human premolar root canal samples had been prepared and sterilized. All dental root samples were inoculated with P.gingivalis bacteria that had been cultured for 24 hours at 37oC. Samples were divided into 4 groups according to irrigant types, group 1 were reuterin isolated from L. reuteri LC 382415 (Indonesian strain) 12,5 ug/ml, group 2 were reuterin isolated from L. reuteri Prodentis (BioGaia, Stockholm, Sweden), group 3 were NaOCl 2,5 % (positive control), group 4 were saline (negative control). Each irrigant types were divided into 2 contact times, 5 minutes and 30 minutes. Subsequently, genes expression quantification were determined by RT-PCR. Data were analyzed using one-way ANOVA with P<0.05 as level of significance.
Results: MIC of reuterin for P. gingivalis were found to be 12.5 μg/mL. Statistically, reuterin 12.5 μg/mL significantly reduced the transcription of fimA, mfa1, and hagB gene expression to form P. gingivalis biofilm in an ex vivo root canal model both at 5 minutes and 30 minutes contact time (P<0.05)
Conclusions: The results indicated that the anti-biofilm activity of reuterin against P. gingivalis may be due to attenuating the virulence genes. Hence, reuterin may develop as a potential source for future endodontic treatment. However, further clinical research is required for concerete conclusions.