IADR Abstract Archives

Probiotic Lactobacillus Reuteri Improves Oral Health of Fixed Orthodontic Patients

Objectives: Present study investigated the effect of probiotic Lactobacillus reuteri consumption on improving the oral health of patients under fixed orthodontic treatment by modulating the virulence gene expression of Porphyromonas gingivalis in dental plaque biofilms.
Methods: This clinical study included 20 subjects (age 18-28 years old) who have used fixed orthodontics appliances for at least 12 months. Subjects received probiotic L. reuteri DSM 17938 and L. reuteri ATCC PTA 5289 Lozenges (BioGaia, Stockholm, Sweden) once daily for 14 days. Clinical data of oral hygiene index score (OHI-s) and papilla bleeding index (PBI) and dental plaque sampling were collected before and after 14 days of probiotic interventions. Dental plaque samples were analyzed using RT-PCR to evaluate the Porphyromonas gingivalis biofilms associated-gene (uspA, hagB, fimA, and mfa1) expressions. All data were statistically analyzed using a paired t-test with p < 0.05 was set as level of significance.
Results: Probiotic L. reuteri lozenges consumption for 14 days improved the oral health of the subjects, as shown by the reduction of OHI score and PBI score significantly (P<0.05). Furthermore, probiotic consumption significant reduced the expressions of uspA, hagB, fimA, and mfa1 genes in P. gingivalis from dental plaque biofilm (P<0.05).
Conclusions: The present study demonstrated that L. reuteri probiotics has the ability to improve oral health by modulating the virulent gene expression of periodontal pathogens. Hence, L. reuteri consumption may develop as an adjunctive periodontal therapy in fixed orthodontic patients. However, long term clinical trials are warranted to obtain further evidence on this possible clinical treatment.

2021 South East Asian Division Meeting (Hong Kong)
Hong Kong
2021
089
Microbiology/Immunology
  • Halim, Louise  ( Trisakti University , Jakarta , DKI Jakarta , Indonesia )
  • Widyarman, Armelia  ( Faculty of Dentistry, Trisakti University , Jakarta , DKI Jakarta , Indonesia )
  • Seneviratne, Chaminda  ( National Dental Center Singapore , Singapore , Singapore )
  • none
    Poster Session
    Oral microbiome, microbiology and immunology II
    Thursday, 12/09/2021 , 12:00PM - 01:00PM