IADR Abstract Archives
Lactobacillus Reuteri Probiotic Consumption Reduced the Inflammatory Responses in Orthodontic Patients
Objectives:
Tooth movement produced by orthodontic braces can lead to periodontal ligament inflammation, which triggers the release of cytokines, such as interleukin-6 (IL-6) and IL-8, as well as tumor necrosis factor-alpha (TNF-α) and matrix metalloprotease-9 (MMP-9). Several studies have demonstrated the oral health benefits of probiotic bacteria. The aim of this study was to analyze the effect of 2 weeks of probiotic consumption on the inflammatory response via an analysis of IL-6, IL-8, MMP-9, and TNF-α concentrations in individuals with fixed orthodontic appliances.
Methods:
Twenty subjects aged 18–24 years who had an orthodontic appliance for at least 12 months consumed probiotic lozenges (BioGaia, Stockholm, Sweden) containing 2 × 108 colony forming units (CFU) of two L. reuteri strains, DSM17938 and ATCC PTA 5289, daily for 2 weeks. Gingival crevicular fluid (GCF) samples were collected from the subjects before and after the probiotic treatment. IL-6, IL-8, MMP-9, and TNF-α concentrations were analyzed by an enzyme-linked immunosorbent assay (ELISA). All the data were analyzed by a paired T-test. A p-value of less than 0.05 was considered statistically significant.
Results: The level of inflammatory responses of IL-6, IL-8, MMP-9, and TNF-α were decreased after probiotic consumption. There was a significant reduction of these inflammatory responses before and 14 days after probiotic consumption in all the subjects (p < 0.05).
Conclusions: The probiotic L. reuteri reduced the level of inflammatory factors (proinflammatory cytokines IL-6, IL-8, MMP, and TNF-α). L. reuteri probiotics can improve the healing process and prevent periodontal disease in patients with fixed orthodontic appliances. Further research is needed to shed light on the other inflammatory factors expressed in patients with fixed orthodontic appliances. In addition, larger sample numbers and different age groups to increase the generalizability of the findings to the population level.
Tooth movement produced by orthodontic braces can lead to periodontal ligament inflammation, which triggers the release of cytokines, such as interleukin-6 (IL-6) and IL-8, as well as tumor necrosis factor-alpha (TNF-α) and matrix metalloprotease-9 (MMP-9). Several studies have demonstrated the oral health benefits of probiotic bacteria. The aim of this study was to analyze the effect of 2 weeks of probiotic consumption on the inflammatory response via an analysis of IL-6, IL-8, MMP-9, and TNF-α concentrations in individuals with fixed orthodontic appliances.
Methods:
Twenty subjects aged 18–24 years who had an orthodontic appliance for at least 12 months consumed probiotic lozenges (BioGaia, Stockholm, Sweden) containing 2 × 108 colony forming units (CFU) of two L. reuteri strains, DSM17938 and ATCC PTA 5289, daily for 2 weeks. Gingival crevicular fluid (GCF) samples were collected from the subjects before and after the probiotic treatment. IL-6, IL-8, MMP-9, and TNF-α concentrations were analyzed by an enzyme-linked immunosorbent assay (ELISA). All the data were analyzed by a paired T-test. A p-value of less than 0.05 was considered statistically significant.
Results: The level of inflammatory responses of IL-6, IL-8, MMP-9, and TNF-α were decreased after probiotic consumption. There was a significant reduction of these inflammatory responses before and 14 days after probiotic consumption in all the subjects (p < 0.05).
Conclusions: The probiotic L. reuteri reduced the level of inflammatory factors (proinflammatory cytokines IL-6, IL-8, MMP, and TNF-α). L. reuteri probiotics can improve the healing process and prevent periodontal disease in patients with fixed orthodontic appliances. Further research is needed to shed light on the other inflammatory factors expressed in patients with fixed orthodontic appliances. In addition, larger sample numbers and different age groups to increase the generalizability of the findings to the population level.