IADR Abstract Archives
A Comparison of Two Methods for the Detection of Circulating Tumour Cells in Patients With Oral Cavity Cancer
Objectives: Circulating tumour cells (CTCs) detected in patient blood samples can be used as diagnostic and prognostic markers offering insights into tumour behaviour and guiding treatment of cancer at an individualised level.
This study aims to investigate which method -filtration by size(ISET®) or in-situ fluorescent immunostaining(i-FISH, Cytelligen®) is more reliable to detect CTCs in patients with oral squamous cell carcinoma(OSCC) undergoing surgical resection.
Methods: Comparison of the numbers of CTCs, circulating tumour micro-emboli (CTMs) and circulating tumour endothelial cells (CTECs) was undertaken in forty clinical samples of ten patients with OSCC undergoing surgical resection determined by ISET®) and i-FISH.
Results: i-Fish was able to detect CTCs in 80% of the samples as compared ISET which only found CTCs in 40% of the samples. No CTM were detected by ISET while i-FISH detected CTM in 12. 5% of the samples. i-FISH analysis also detected CTECs in 20 out of 40 samples.
Conclusions: These results show that i-FISH analysis provided more detailed information regarding circulating cells when compared to ISET®. Cell clusters and circulating endothelial cells could be detected using i-FISH and these species could provide insight into longer-term patient outcomes. These results also highlight that technologies capable of detecting circulating aneuploidic cells (i-FISH) can more accurately detect CTCs. With proven prognostic relevance in adenocarcinomas, accurate enumeration of CTCs, CTMs and CTECs may be a clinically useful tool in the management of OSCC and may aid in the reduction of false negative diagnoses.
This study aims to investigate which method -filtration by size(ISET®) or in-situ fluorescent immunostaining(i-FISH, Cytelligen®) is more reliable to detect CTCs in patients with oral squamous cell carcinoma(OSCC) undergoing surgical resection.
Methods: Comparison of the numbers of CTCs, circulating tumour micro-emboli (CTMs) and circulating tumour endothelial cells (CTECs) was undertaken in forty clinical samples of ten patients with OSCC undergoing surgical resection determined by ISET®) and i-FISH.
Results: i-Fish was able to detect CTCs in 80% of the samples as compared ISET which only found CTCs in 40% of the samples. No CTM were detected by ISET while i-FISH detected CTM in 12. 5% of the samples. i-FISH analysis also detected CTECs in 20 out of 40 samples.
Conclusions: These results show that i-FISH analysis provided more detailed information regarding circulating cells when compared to ISET®. Cell clusters and circulating endothelial cells could be detected using i-FISH and these species could provide insight into longer-term patient outcomes. These results also highlight that technologies capable of detecting circulating aneuploidic cells (i-FISH) can more accurately detect CTCs. With proven prognostic relevance in adenocarcinomas, accurate enumeration of CTCs, CTMs and CTECs may be a clinically useful tool in the management of OSCC and may aid in the reduction of false negative diagnoses.