IADR Abstract Archives
The Periodontitis Treating and Microbiota Changing Effects of Sapindus Mukorossi Seed oil on Ligature-Induced Periodontitis rat Model
Objectives: It was reported that Sapindus mukorossi (S. mukorossi) seed oil exhibited anti-bacterial and anti-inflammation effects. However, the possibility for applying this plant oil to treat oral disease was not reported previously. The aim of this study was to evaluate the effects of S. mukorossi seed oil on experimental periodontitis in rats. The changing of microbial community structure was analyzed using DNA profile technique.
Methods: The ligature-induced periodontitis model was carried out in this study. Twenty rats were divided into four groups (0day, 7day, 14day and 21day). Ligatures were placed on the maxillary second molar bilaterally with one side was ligated without S. mukorossi seed oil, and the other side was set as S. mukorossi seed oil positive. Rats were sacrifice on days 0, 7, 14, and 21 after ligature placement. The periodontal bone lose was evaluated with micro-computed tomography and histological images with hematoxylin and eosin staining. After the animal was scarified, the ligatures were collected and 16s RNA gene analyses was performed to observe the effect of S. mukorossi seed oil on microbial community structure.
Results: In the 14-day group, the S. mukorossi seed oil shows significant inhibion efficiency of alveolar bone loss when compared to negative control samples. During the three-week observation, the microbial community structure was significantly different between S. mukorossi seed oil treated and untreated groups. In addition, 16s RNA gene analyses demonstrated that the proportion of periodontal-related bacterium (Bacteroidaceae, Eubacteriaceae, Peptostreptococcaceae, Pasteurellaceae, Pseudomonadaceae) were reduced.
Conclusions: Overall, the present results show that topical application of S. mukorossi seed oil can reduce inflammation and bone resorption in ligature-induced periodontitis model. According to this result, it is reasonable to suggest that S. mukorossi seed oil could be a potential material to prevent deterioration of periodontal disease.
Methods: The ligature-induced periodontitis model was carried out in this study. Twenty rats were divided into four groups (0day, 7day, 14day and 21day). Ligatures were placed on the maxillary second molar bilaterally with one side was ligated without S. mukorossi seed oil, and the other side was set as S. mukorossi seed oil positive. Rats were sacrifice on days 0, 7, 14, and 21 after ligature placement. The periodontal bone lose was evaluated with micro-computed tomography and histological images with hematoxylin and eosin staining. After the animal was scarified, the ligatures were collected and 16s RNA gene analyses was performed to observe the effect of S. mukorossi seed oil on microbial community structure.
Results: In the 14-day group, the S. mukorossi seed oil shows significant inhibion efficiency of alveolar bone loss when compared to negative control samples. During the three-week observation, the microbial community structure was significantly different between S. mukorossi seed oil treated and untreated groups. In addition, 16s RNA gene analyses demonstrated that the proportion of periodontal-related bacterium (Bacteroidaceae, Eubacteriaceae, Peptostreptococcaceae, Pasteurellaceae, Pseudomonadaceae) were reduced.
Conclusions: Overall, the present results show that topical application of S. mukorossi seed oil can reduce inflammation and bone resorption in ligature-induced periodontitis model. According to this result, it is reasonable to suggest that S. mukorossi seed oil could be a potential material to prevent deterioration of periodontal disease.