IADR Abstract Archives
Mechanical Force Regulates Induced Pluripotent Stem Cell Behaviors
Objectives: Mechanical force regulates various cell behaviors in stem cells. The present study aims to investigate the effect of mechanical force on murine induced pluripotent stem cells (miPS).
Methods: Adhering retinoic acid-treated miPS were treated with intermittent compressive force (ICF) in serum free culture medium (SFM) for 24 hours. The mRNA expression was determined by RNA sequencing analysis and real-time polymerase chain reaction. Immunofluorescence staining was used to investigate protein expression. Cell cycle analysis and apoptosis assay were performed to determine cell proliferation and death.
Results: ICF induced cell cycle while reduced apoptotic cells and increased genes involved in cell cycle and P53 pathway, Ccnd1, Cdk6 and Ccng1. The ICF also upregulated transforming growth factor b1 (Tgfb1) expression in both mRNA and protein levels and pretreatment with TGF-b inhibitor (SB431542) prior to the ICF application abolished the ICF increased Ccnd1and Cdk6 expression.
Conclusions: ICF promotes proliferation and cell cycle while inhibits apoptosis of miPS occurred via the TGF-b pathway.
Methods: Adhering retinoic acid-treated miPS were treated with intermittent compressive force (ICF) in serum free culture medium (SFM) for 24 hours. The mRNA expression was determined by RNA sequencing analysis and real-time polymerase chain reaction. Immunofluorescence staining was used to investigate protein expression. Cell cycle analysis and apoptosis assay were performed to determine cell proliferation and death.
Results: ICF induced cell cycle while reduced apoptotic cells and increased genes involved in cell cycle and P53 pathway, Ccnd1, Cdk6 and Ccng1. The ICF also upregulated transforming growth factor b1 (Tgfb1) expression in both mRNA and protein levels and pretreatment with TGF-b inhibitor (SB431542) prior to the ICF application abolished the ICF increased Ccnd1and Cdk6 expression.
Conclusions: ICF promotes proliferation and cell cycle while inhibits apoptosis of miPS occurred via the TGF-b pathway.