IADR Abstract Archives
Enhancing Resin-Dentin Bond Durability Using a Novel Mussel-Inspired Monomer
Objectives: To assess the effect of N-(3,4-dihydroxyphenethyl)methacrylamide (DMA) as a functional monomer in dentin bonding.
Methods: The application of DMA as a functional monomer to cross-link with dentin collagen and polymerize with adhesive was evaluated using transmission electron microscopy (TEM), attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy, and atomic force microscopy (AFM) via Peakforce QNM mode. After validating the influence of DMA on collagen and adhesive separately, the overall performance of DMA/ethanol solution (1, 5, and 10 mmol/L) as a primer in dentin bonding was examined using micro-tensile bond strength (MTBS), fracture pattern observation, and nanoleakage evaluation both immediately and after 10,000 times thermocycling aging. Besides, the inhibitory effect of DMA on endogenous matrix metalloproteinases (MMPs) was evaluated by in situ zymography using confocal laser scanning microscopy (CLSM) and the cytotoxicity of DMA was evaluated using cell counting kit-8 (CCK-8).
Results: DMA successfully cross-linked with dentin collagen via non-covalent bonds and had no influence on the polymerization and mechanical properties of the adhesive. After 10,000 times thermocycling aging, the MTBS and nanoleakage expression of the DMA-treated groups showed no significant change compared with their immediate values (p > 0.05). In situ zymography revealed reduced endogenous proteolytic activities after the application of DMA, and no cytotoxicity effect was observed for DMA concentration up to 25 μmol/L.
Conclusions: DMA could be used as a novel, biocompatible functional monomer in dentin bonding. It served as a bridge connecting the adhesive and dentin collagen network, unifying them as one whole structure to resist hydrolytic and protease degradation, thereby enhancing the durability of resin-dentin bond.
Methods: The application of DMA as a functional monomer to cross-link with dentin collagen and polymerize with adhesive was evaluated using transmission electron microscopy (TEM), attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy, and atomic force microscopy (AFM) via Peakforce QNM mode. After validating the influence of DMA on collagen and adhesive separately, the overall performance of DMA/ethanol solution (1, 5, and 10 mmol/L) as a primer in dentin bonding was examined using micro-tensile bond strength (MTBS), fracture pattern observation, and nanoleakage evaluation both immediately and after 10,000 times thermocycling aging. Besides, the inhibitory effect of DMA on endogenous matrix metalloproteinases (MMPs) was evaluated by in situ zymography using confocal laser scanning microscopy (CLSM) and the cytotoxicity of DMA was evaluated using cell counting kit-8 (CCK-8).
Results: DMA successfully cross-linked with dentin collagen via non-covalent bonds and had no influence on the polymerization and mechanical properties of the adhesive. After 10,000 times thermocycling aging, the MTBS and nanoleakage expression of the DMA-treated groups showed no significant change compared with their immediate values (p > 0.05). In situ zymography revealed reduced endogenous proteolytic activities after the application of DMA, and no cytotoxicity effect was observed for DMA concentration up to 25 μmol/L.
Conclusions: DMA could be used as a novel, biocompatible functional monomer in dentin bonding. It served as a bridge connecting the adhesive and dentin collagen network, unifying them as one whole structure to resist hydrolytic and protease degradation, thereby enhancing the durability of resin-dentin bond.
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