IADR Abstract Archives

MicroRNA-302a-3p Delivery by Surface-Modified Hydroxyapatite Nanoparticle and 3D-Printed TCP/HA Scaffold Promoted Osteogenic Differentiation

Objectives: Bone tissue engineering (BTE) is a preferable approach for critical-sized bone defects to restore functions and esthetics. This study aims to demonstrate the custom-shaped Tricalcium phosphate/Hydroxyapatite (TCP/HA) scaffold with bioactive molecules for promoting cell proliferation and differentiation.
Methods: The macro- and microstructure of the scaffold are designed and printed using 3D-printing technology. The scaffold is incorporated with hydroxyapatite nanoparticles presenting cationic functional molecules 3-aminopropyltriethoxysilane to carry microRNA (HA-NPs-APTES-miR) as the bioactive molecules. Biocompatibility of the TCP/HA scaffold carrying HA-NPs-APTES-miR were tested in human primary osteoblast (HOB) and osteosarcoma cells (HOS) by using resazurin assay. Dispersion and the uptake of HA-NPs-APTES-miR was visualized by fluorescence microscopy. The delivery and regulation of targeted mRNAs was demonstrated by qPCR. The use of TCP/HA scaffolds carrying HA-NPs-ATES-miR for bone regeneration was demonstrated in the C57BL/6 mouse model.
Results: TCP/HA scaffolds carrying HA-NPs-ATES-miR were biocompatible, and desired physical properties are demonstrated. HA-NPs-APTES are equally dispersed on the scaffolds, and intracellular particles are visualized at 24 hours. After a 5 day incubation on the scaffolds, miRNA302a-3p expression in HOS and HOB were significantly up-regulated by 1.4x105- and 1.41x106-fold respectively. In consistent with the decrease of its target genes, suggesting the successful delivery and the functions of miRNA302a-3p in HOB and HOS cultures. The down-regulation of COUP-TFII as the target mRNA resulted in upregulation of the osteogenic gene, RUNX2, in HOS and HOB by 8.6 and 6.22 fold, respectively. The in-vitro results were then confirmed in the in-vivo model to demonstrate bone regeneration by micro tomography and histological staining.
Conclusions: The successful delivery of miRNA-302a-3p by HA-NPs-APTES and TCP/HA scaffold is demonstrated in both in-vitro and in-vivo models. The HA-NPs-APTES and TCP/HA printing provides a custom-shaped scaffold design to fit the defects, which could be promising as a future therapeutic approach for BTE.

2021 South East Asian Division Meeting (Hong Kong)
Hong Kong
2021
001
Dental Materials 5: Biocompatibility, Bioengineering and Biologic Effects of Materials
  • Limlawan, Pirawish  ( Faculty of Dentistry, Chulalongkorn University , Pathumwan , Bangkok , Thailand ;  Faculty of Dentistry, Chulalongkorn University , Pathumwan , Bangkok , Thailand )
  • Durual, Stéphane  ( University Clinic of Dental Medicine, University of Geneva , Geneva , Switzerland )
  • Vacharaksa, Anjalee  ( Faculty of Dentistry, Chulalongkorn University , Pathumwan , Bangkok , Thailand ;  Faculty of Dentistry, Chulalongkorn University , Pathumwan , Bangkok , Thailand )
  • NONE
    Royal Golden Jubilee (RGJ) Ph.D. Programme, Thailand Research Fund.
    Oral Session
    Dental materials and biomaterials I
    Wednesday, 12/08/2021 , 10:15AM - 11:45AM