IADR Abstract Archives
Synthesis and Characterization of a Novel Calcium Strontium Silicate for Potential Dental Applications
Objectives: To synthesize and characterize calcium strontium silicate ceramic, as a new material for potential use in various restorative and regenerative dental treatments.
Methods: Calcium silicate (CS), and calcium strontium silicate (CSR) were synthesized by the sol-gel method. The synthesized powders were characterized using x-ray diffraction (XRD). Setting time was measured using the Gilmore needle indentation technique. The compressive strength was measured using the universal testing machine at crosshead speed of 1.0 mm/min until fracture occurred. The in vitro apatite-formation was evaluated by SEM and EDX, and FTIR. The NIH/3T3 cells viability against the synthesized ceramics was tested using MTT assay. The osteogenic differentiation of HDPSC was evaluated by measuring the alkaline phosphatase activity (ALP), and mineralization by alizarin red stain (ARS). The ions release of Ca, Sr, and Si were measured using inductive coupled plasma-optical emission spectroscopy (ICP-OES). The independent t-test was used to analyze setting time, compressive strength, and ARS results. One-way ANOVA was used to analyse ALP and cell viability results. The Bonferroni post hoc test was used to establish significance (p < 0.05)
Results: CSR was successfuly synthesized, and phase identification was confirmed with XRD. The initial and final setting times were significantly shorter in CSR 5 ± 0,75 min, 29 ± 1.9 min than CS 8 ± 0.77 min, 31 ± 1.39 min, respectively. No significant difference was found between the compressive strength of CS and CSR (p > 0.05). CSR demonstrated higher apatite forming-ability and cell viability than CS. The ALP activity was significantly higher in CSR 1.16 ± 0.12 than CS 0.92 ± 0.15 after 14 d of culture (p<0.05). ARS showed higher mineralization in CSR than CS after 14 and 21 d culture times.
Conclusions: Calcium strontium silicate was successfully synthesized by the sol-gel method. CSR demonstrated enhanced biocompatibility, bioactivity and physicomechanical properties than CS. CSR could be a promising material for vital pulp therapies and might aid in the prognosis of regenerative endodontic treatments.
Methods: Calcium silicate (CS), and calcium strontium silicate (CSR) were synthesized by the sol-gel method. The synthesized powders were characterized using x-ray diffraction (XRD). Setting time was measured using the Gilmore needle indentation technique. The compressive strength was measured using the universal testing machine at crosshead speed of 1.0 mm/min until fracture occurred. The in vitro apatite-formation was evaluated by SEM and EDX, and FTIR. The NIH/3T3 cells viability against the synthesized ceramics was tested using MTT assay. The osteogenic differentiation of HDPSC was evaluated by measuring the alkaline phosphatase activity (ALP), and mineralization by alizarin red stain (ARS). The ions release of Ca, Sr, and Si were measured using inductive coupled plasma-optical emission spectroscopy (ICP-OES). The independent t-test was used to analyze setting time, compressive strength, and ARS results. One-way ANOVA was used to analyse ALP and cell viability results. The Bonferroni post hoc test was used to establish significance (p < 0.05)
Results: CSR was successfuly synthesized, and phase identification was confirmed with XRD. The initial and final setting times were significantly shorter in CSR 5 ± 0,75 min, 29 ± 1.9 min than CS 8 ± 0.77 min, 31 ± 1.39 min, respectively. No significant difference was found between the compressive strength of CS and CSR (p > 0.05). CSR demonstrated higher apatite forming-ability and cell viability than CS. The ALP activity was significantly higher in CSR 1.16 ± 0.12 than CS 0.92 ± 0.15 after 14 d of culture (p<0.05). ARS showed higher mineralization in CSR than CS after 14 and 21 d culture times.
Conclusions: Calcium strontium silicate was successfully synthesized by the sol-gel method. CSR demonstrated enhanced biocompatibility, bioactivity and physicomechanical properties than CS. CSR could be a promising material for vital pulp therapies and might aid in the prognosis of regenerative endodontic treatments.