IADR Abstract Archives

Fabricating Multilayered Magnetic-Nanoparticles Loading With DNase/Vancomycin for Elimination of Multispecies-Biofilm

Objectives: The objective was to design novel multi-layered magnetic nanoparticles (ML-MNPs) loading with DNase and vancomycin for the elimination of multispecies biofilm.
Methods: A core-layer of ML-MNPs comprising of iron-oxide MNPs (IO-core), was synthesized by the co-precipitation method to enhance the biofilm penetration of ML-MNPs with external magnetic force. Further coatings were applied over IO-core with the heavy-metal-based layers (Ag & MoS2 NPs) through the solvent-evaporation method. Thus, the external layers comprising of Ag/MoS2 NPs might act as the antimicrobial-nanozyme layers. DNase and vancomycin were loaded onto ML-MNPs through physical deposition and adsorption into its outermost layer comprising of nonporous MoS2 NPs. Biofilms of S. mutans or E. faecalis (or both) were grown in brain-heart-infusion broth (BHI) for 4 days followed by treatment with the ML-MNPs for 24 h.
Results: The coatings of the Ag (200 nm), and ultra-small MoS2 (20 nm) were finally assembled as outer layers of ML-MNPs to become Ag-Fe3O4@MoS2 MNPs (3-5 µm). The ML-MNPs (nanozymes) selectively interfered with the bacterial metabolism, proliferation, and adversely affected the biofilm integrity. Whereas, the treatment of bacterial biofilm with the blank-solution, empty-MNPs, free DNase/vancomycin (alone or in combinations) reduced the overall thickness to a range of 10 to 45 µm, but it did not achieve critical levels of biofilm inhibition. However, loading DNase/vancomycin onto the nanoporous MoS2-layer of the ML-MNPs enhanced the nanochannel creation through the various layers of the biofilm. Therefore, vancomycin/DNase-loaded ML-MNPs reduced the thickness of 72-h, oral-bacterial biofilm from 48 µm to a range of 0 to 4 µm, with 96%-100% bactericidal-effects.
Conclusions: Vancomycin/DNase-loaded ML-MNPs are effective in killing S. mutans or E. faecalis, reducing the biofilm thickness.

2021 South East Asian Division Meeting (Hong Kong)
Hong Kong
2021
065
Dental Materials 3: Metal-based Materials and Other Materials
  • Baig, Mirza Muhammad Faran Ashraf  ( The University of Hong Kong , Hong Kong SAR , China )
  • Zhang, Chengfei  ( The University of Hong Kong , Hong Kong SAR , China )
  • "None"
    Platform Technology Funding from The University of Hong Kong to C. Zhang.
    Oral Session
    Dental materials and biomaterials III
    Wednesday, 12/08/2021 , 03:15PM - 04:45PM