IADR Abstract Archives
Characteristic of Dental Pulp Stromal Cells From Cleft Lip Palate Patients
Objectives: Cleft lip and palate (CL/P) is a complex multifactorial disorder and one of the most common congenital orofacial malformations. Alveolar bone defect reconstruction using mesenchymal stromal cells (MSCs) have been previously reported. However, the characteristics of MSCs from CL/P patients was unclear. Understanding of its characteristics is of importance for their potential clinical use. The present study aimed to evaluate the characteristics of dental pulp stromal cells (DPSCs) from non-syndromic cleft lip and palate patients (NSCL/P) in comparison to healthy matched controls.
Methods: DPSCs were isolated from 12 teeth. MSCs immunotyping, cell proliferation capacity and osteogenic differentiation was tested. Human Homeobox (HOX) and osteogenesis genes expression were analyzed with RT2 Profiler PCR Array.
Results: Cell proliferation of DPSCs from NSCL/P at day 1, 3 and 7 was comparable with control (p>0.05). Similarly, cell population expressing MSCs specific markers of CD73, CD90 and CD106 was comparable between the two groups tested. Population doubling time was 1.15±0.27 and 1.35±0.31 for DPSCs from NSCL/P and healthy control, respectively (p=0.18). No different was found in the ALP, Col1A1, and Runx2 mRNA expression following 21 days of osteogenic induction. RT2 Profiler PCR Array of osteogenesis genes revealed significant differences in the expression of IGF1, Col10A1, FGFR1, MMP2 and PHEX genes. While gene differentially expressed in the two group tested for HOX showed EN1, HOXC9, SHOX and CUX1 genes. The fold regulation threshold for osteogenesis and HOX genes was 2 (p<0.05).
Conclusions: Differences exist between homeobox and osteogenesis genes between DPSCs from NSCL/P patients and their matched controls. Nonetheless, in vitro DPSCs culture showed comparable results with regards to cell proliferation and osteogenic differentiation. The present study highlights the potential use of DPSCs from NSCL/P patients for bone defect reconstruction.
Methods: DPSCs were isolated from 12 teeth. MSCs immunotyping, cell proliferation capacity and osteogenic differentiation was tested. Human Homeobox (HOX) and osteogenesis genes expression were analyzed with RT2 Profiler PCR Array.
Results: Cell proliferation of DPSCs from NSCL/P at day 1, 3 and 7 was comparable with control (p>0.05). Similarly, cell population expressing MSCs specific markers of CD73, CD90 and CD106 was comparable between the two groups tested. Population doubling time was 1.15±0.27 and 1.35±0.31 for DPSCs from NSCL/P and healthy control, respectively (p=0.18). No different was found in the ALP, Col1A1, and Runx2 mRNA expression following 21 days of osteogenic induction. RT2 Profiler PCR Array of osteogenesis genes revealed significant differences in the expression of IGF1, Col10A1, FGFR1, MMP2 and PHEX genes. While gene differentially expressed in the two group tested for HOX showed EN1, HOXC9, SHOX and CUX1 genes. The fold regulation threshold for osteogenesis and HOX genes was 2 (p<0.05).
Conclusions: Differences exist between homeobox and osteogenesis genes between DPSCs from NSCL/P patients and their matched controls. Nonetheless, in vitro DPSCs culture showed comparable results with regards to cell proliferation and osteogenic differentiation. The present study highlights the potential use of DPSCs from NSCL/P patients for bone defect reconstruction.