IADR Abstract Archives
Oral Microbiome Characterization of Urban and Rural Healthy Women in Indonesia
Objectives: The oral microbiome studies performed in Indonesia especially in between urban and rural area are remain underrepresented. According to Indonesia Basic Health Research (RISKESDAS) 2018, most of noncommunicable diseases, showed an increasing trend compared to the previous report in 2013. The prevalence of diabetes, heart disease, hypertension and obesity are higher in the urban area than in rural area. All rates are found higher in the females than males. The aim of this pilot study was to examine the oral microbiome charcterization isolated from the tongue biofilm of urban and rural Indonesian healthy women with the level of oral hygiene.
Methods: Twenty women aged 21-47 years old from West Jakarta, DKI Jakarta’s residents (n=10) as representative of urban area, and Ende, Nangapanda, East Nusa Tenggara’s residents (n=10) as representative of rural area were voluntary participated in this study. The participants were evaluated by the Simplified Oral Hygiene Index (OHI-S) according to the criteria of Greene & Vermillion and divided into three groups. High-throughput DNA sequencing were performed on an Illumina iSeq 100 platform.
Results: This study revealed phylum Firmicutes was the most abundant in both urban and rural area. However, genus Prevotella was abundant in urban area while genus Streptococcus was the most abundant in rural area. At species level, Leptotrichia wadei, Prevotella melaninogenica, and Prevotella jejuni were dominated in urban area, while Acinetobacter junii Actinomyces odontolyticus, and Rothia mucilaginosa were found dominantly in rural area.
Conclusions: This is the first study demonstrating the characterization of oral microbiome in the Indonesian women in urban and rural area. The microbial communities display unique characterization between the urban area and rural area. The specific characterization of Indonesian women microbiota are likely linked to the host diet, cultural habits and socioeconomic status as well as the geographic location or population studied.
Methods: Twenty women aged 21-47 years old from West Jakarta, DKI Jakarta’s residents (n=10) as representative of urban area, and Ende, Nangapanda, East Nusa Tenggara’s residents (n=10) as representative of rural area were voluntary participated in this study. The participants were evaluated by the Simplified Oral Hygiene Index (OHI-S) according to the criteria of Greene & Vermillion and divided into three groups. High-throughput DNA sequencing were performed on an Illumina iSeq 100 platform.
Results: This study revealed phylum Firmicutes was the most abundant in both urban and rural area. However, genus Prevotella was abundant in urban area while genus Streptococcus was the most abundant in rural area. At species level, Leptotrichia wadei, Prevotella melaninogenica, and Prevotella jejuni were dominated in urban area, while Acinetobacter junii Actinomyces odontolyticus, and Rothia mucilaginosa were found dominantly in rural area.
Conclusions: This is the first study demonstrating the characterization of oral microbiome in the Indonesian women in urban and rural area. The microbial communities display unique characterization between the urban area and rural area. The specific characterization of Indonesian women microbiota are likely linked to the host diet, cultural habits and socioeconomic status as well as the geographic location or population studied.