IADR Abstract Archives
Acemannan Modulates Resin-Modified Glass-Ionomer Cement Physical Properties, Fluoride-Release and Biocompatibility
Objectives: To investigate the depth of cure, flexural strength, fluoride ion release, and cytotoxicity of an acemannan-containing resin modified glass ionomer cement (aceRU-HBM1).
Methods: Acemannan (3, 5, or 10%) was added into the resin modified glass ionomer cement (aceRU-HBM1), with VitraBond (VB) as control. The depth of cure and flexural strength were determined per ISO. The cumulative fluoride release was measured by suspending each sample in deionized water. The solutions were collected and replaced at day 1, 3, 7, 14, and 28. Primary human dental pulp cells were cultured in the conditioned medium from each specimen, with growth media used as a control. Cytotoxicity was determined using an MTT assay.
Results: The aceRU-HBM1 samples met the ISO requirements for depth of cure and flexural strength. The 10% aceRU-HBM1 significantly reduced the depth of cure compared with RU-HBM1, 3%, and 5% aceRU-HBM1 (p < 0.05). There was no significant difference in flexural strength between the RU-HBM1 and aceRU-HBM1 groups (p>0.05). The cumulative fluoride ion release in the RU-HBM1 and 5% aceRU-HBM1 groups were significantly higher compared with VB at day 14 and 28 (p<0.05). The MTT assay revealed that the 5% and 10% aceRU-HBM1 conditioned media significantly increased cell viability at 24 h, while the VB conditioned media reduced cell viability (p < 0.05).
Conclusions: The ace-RU-HBM1 samples met the ISO requirements for depth of cure and flexural strength. RU-HBM1 and aceRU-HBM1 released fluoride ion up to 28 days. 5% and 10% aceRU-HBM1 induced pulp cell proliferation.
Methods: Acemannan (3, 5, or 10%) was added into the resin modified glass ionomer cement (aceRU-HBM1), with VitraBond (VB) as control. The depth of cure and flexural strength were determined per ISO. The cumulative fluoride release was measured by suspending each sample in deionized water. The solutions were collected and replaced at day 1, 3, 7, 14, and 28. Primary human dental pulp cells were cultured in the conditioned medium from each specimen, with growth media used as a control. Cytotoxicity was determined using an MTT assay.
Results: The aceRU-HBM1 samples met the ISO requirements for depth of cure and flexural strength. The 10% aceRU-HBM1 significantly reduced the depth of cure compared with RU-HBM1, 3%, and 5% aceRU-HBM1 (p < 0.05). There was no significant difference in flexural strength between the RU-HBM1 and aceRU-HBM1 groups (p>0.05). The cumulative fluoride ion release in the RU-HBM1 and 5% aceRU-HBM1 groups were significantly higher compared with VB at day 14 and 28 (p<0.05). The MTT assay revealed that the 5% and 10% aceRU-HBM1 conditioned media significantly increased cell viability at 24 h, while the VB conditioned media reduced cell viability (p < 0.05).
Conclusions: The ace-RU-HBM1 samples met the ISO requirements for depth of cure and flexural strength. RU-HBM1 and aceRU-HBM1 released fluoride ion up to 28 days. 5% and 10% aceRU-HBM1 induced pulp cell proliferation.