IADR Abstract Archives

Silver Diamine Fluoride Application in Deep Carious Lesions: in Vitro

Objectives: This study aimed to evaluate depth and pattern of silver penetration in deep carious lesions in permanent teeth treated with 38% silver diamine fluoride (SDF).
Methods: Six extracted human permanent teeth with carious lesion extending ≥ inner third of dentin were randomly divided into 2 groups, according to deep caries removal technique. Peripheral soft carious infected dentin was completely removed at DEJ in both groups. Group A: soft carious infected dentin was left pulpally, while Group B: leathery to soft carious infected dentin was left pulpally. Then, both groups were treated with 38% SDF for 3 minutes and rinsed with distilled water for 10 seconds. Before and after SDF application, the samples were scanned using micro-CT (voxel size 18.5 µm) to measure mineral density. Two parameters were calculated, which were estimated lesion depth and estimated penetration depth. The samples were then fixed with 2.5% glutaraldehyde, 0.1% osmium tetroxide, and dehydrated in ascending ethanol concentrations. The samples were embeded in clear resin and sectioned through the region of interest. The pattern of silver particles penetration was assessed by FE-SEM and EDS-point scan.
Results: The micro-CT analysis showed that the estimated silver penetration depth was greater than the estimated lesion depth (200-300 µm). Depending on mineral density and remaining dentin thickness, silver can penetrate into underneath dentin or dental pulp in both groups. FE-SEM images showed that silver particles densely deposited at superficial lesions and occluded in dentinal tubules. EDS-point scan confirmed that increased mineral density was a result from silver particles precipitation.
Conclusions: The silver penetration depth is positively associated with the lesion depth. SDF is a promising conservative method for deep carious lesion management.

2020 South East Asia Division Meeting (Virtual)

2020
P048
Cariology Research-Fluoride & Ca-based Products
  • Manuschai, Jutharat  ( Faculty of Dentistry, Prince of Songkla University , Hat Yai , Songkhla , Thailand )
  • Talungchit, Supitcha  ( Faculty of Dentistry, Prince of Songkla University , Hat Yai , Songkhla , Thailand )
  • Naorungroj, Supawadee  ( Faculty of Dentistry, Prince of Songkla University , Hat Yai , Songkhla , Thailand )
  • NONE
    Poster Session
    Poster 4
    Friday, 11/27/2020 , 03:15PM - 03:45PM