IADR Abstract Archives

Reuterin Inhibits Candida Albicans and Staphylococcus Aureus Biofilms on Dentures

Objectives: Denture stomatitis is the commonest debilitating problem among denture users worldwide. Currently, patients have to undergo a course topical drugs. However, immunocompromised patients may require systemic antimicbial agents. One of the alternative treatment could be natural probiotics. Lactobacillus reuteri, best known as a probiotic bacteria, produces antimicrobial substance called reuterin. The aim of the present study was to evaluate the effect of reuterin isolated from probiotic L. reuteri on C. albicans and S. aureus biofilms using in-vitro model of denture stomatitis.
Methods: S. aureus were cultured in BHI whereas C. albicans were cultured in SDB at 37 °C in aerobic environment. L. reuteri were cultured in MRS for 24 h, at 37 °C. Firslty, minimum inhibitory concentrations (MIC) of reuterin on planktonic C. albicans and S. aureus were evaluated. Thereafter, standard preventive and therapeutic assays were performed on biofilms grown on denture acrylic plates. Effect of reuterin on biofilms were quantified by crystal violet staining, colony counting and qPCR method and the experimental data were analysed by appropritate statistical tests.
Results: MICs of reuterin against planktonic S. aureus and C. albicans were 100 µg/ml and 25 µg/ml respectively (p< 0.05). Interestingly, reuterin exhibited anti-biofilm activity against S.aureus and C.albicans at 100 µg/ml and eradicate the biofilms by 24 h incubation (p< 0.05). Moreover, reuterin was also effective against monospecies biofilms and mixed species biofilms on acrylic resin plates.
Conclusions: This pioneering study has demonstrated that reuterin has anti-biofilm activity against C.albicans and S.aureus mixed-species biofilms in a model of denture stomatitis. Hence, the new data will provide a basis to develop reuterin as a probiotic-based natural solution for denture stomatitis in future.

2020 South East Asia Division Meeting (Virtual)

2020
S023
Cariology Research-Microbiological Studies/Biofilm
  • Maukar, Thalia  ( Trisakti University Faculty of Dentistry , Jakarta , DKI Jakarta , Indonesia )
  • Irma, Heidi  ( Trisakti University Faculty of Dentistry , Jakarta , DKI Jakarta , Indonesia )
  • Widyarman, Armelia  ( Faculty of Dentistry, Trisakti University , Jakarta , DKI Jakarta , Indonesia )
  • Seneviratne, Chaminda  ( National Dental Center Singapore , Singapore , Singapore )
  • NONE
    Oral Session
    Oral 2
    Friday, 11/27/2020 , 01:00PM - 03:00PM