IADR Abstract Archives
CPP-ACP Varnish Treatment Effect on Oral Microbiome Metagenomic Profile in Children
Objectives: The oral microbiome plays major roles in many oral diseases, including early childhood caries. Caries is associated with alterations of the oral microbiome, including changes in microbial composition and the metabolic functional profile. A topical application of casein phosphopeptide–amorphous calcium phosphate (CPP–ACP) containing fluoride can reduce oral pathogens such as Streptococcus mutansin children’s saliva and the risk of dental caries. The aim of this study was to investigate the efficacy of a CPP–ACP varnish on the plaque microbiome metagenomic profilein children and its potential role in the prevention of dental caries.
Methods: This preliminary study included 10 children aged 8–9 years. The children received topical fluoride CPP–ACP once a week for one month.Plaque samples were collected from 10 children before and after the procedures. Genomic DNA extraction was performed using a Geneaid Presto Buccal Swab gDNA Extraction Kit. Sequencing was performed using an iSeq 100 NGS System.
Results: We discovered several unique bacteria that were significantly different between pre- and post-treatment groups, such as over-abundance of Gemella haemolysans, Granulicatella elegans, andSchwartzia succinivoransafter CPP-ACP treatment, and significantly less abundant of Lautropia mirabilisin post-treatment groups (p< 0.05).G. elegans and G. haemolysansare bacteria usually found in healthy human oral. These bacteria are usually fastidious in growth and difficult in identification, therefore its clinical importance is commonly overlooked, but could become potential biomarkers of successful treatment.
Conclusions: The plaque microbiome in the oral cavity of children aged 8–9 years is a highly heterogeneous ecosystem. Microbial composition changes were observed after CPP–ACP treatment. The abundance and diversity of the oral microbiome differed between the pre- and posttreatment plaque sample groups. Using culture-independent techniques could discover new potential biomarkers for evaluating the efficacy of dental treatment, especially using CPP-ACP.
Methods: This preliminary study included 10 children aged 8–9 years. The children received topical fluoride CPP–ACP once a week for one month.Plaque samples were collected from 10 children before and after the procedures. Genomic DNA extraction was performed using a Geneaid Presto Buccal Swab gDNA Extraction Kit. Sequencing was performed using an iSeq 100 NGS System.
Results: We discovered several unique bacteria that were significantly different between pre- and post-treatment groups, such as over-abundance of Gemella haemolysans, Granulicatella elegans, andSchwartzia succinivoransafter CPP-ACP treatment, and significantly less abundant of Lautropia mirabilisin post-treatment groups (p< 0.05).G. elegans and G. haemolysansare bacteria usually found in healthy human oral. These bacteria are usually fastidious in growth and difficult in identification, therefore its clinical importance is commonly overlooked, but could become potential biomarkers of successful treatment.
Conclusions: The plaque microbiome in the oral cavity of children aged 8–9 years is a highly heterogeneous ecosystem. Microbial composition changes were observed after CPP–ACP treatment. The abundance and diversity of the oral microbiome differed between the pre- and posttreatment plaque sample groups. Using culture-independent techniques could discover new potential biomarkers for evaluating the efficacy of dental treatment, especially using CPP-ACP.