IADR Abstract Archives
A Comparison of MicroRNA Profiles Between rat Incisors and Molars
Objectives: To demonstrate differential microRNA profiles in rodent incisor and molar of the Sprague–Dawley rats
Methods: To study the role of miRNA, we extracted total RNA from the dental pulp tissue collected from the apical part, coronal part of incisors and molars, and compared the expression of miRNAs.
Results: RT-PCR array demonstrated 6 miRNAs (miR-32-5p, 885-5p, 665, 338-3p, 663a, 200a-3p) in apical part and 4 miRNAs (miR-32-5p, 665, 338-3p, 663a) from coronal part are significantly lower than molar group whereas miR-23a-3p is the only one that has significantly increase in both apical and coronal part compare to the molars group.
Conclusions: The results suggested that these miRNAs may have a role in mineralization process during prolonged crown formation of rodent incisors
Methods: To study the role of miRNA, we extracted total RNA from the dental pulp tissue collected from the apical part, coronal part of incisors and molars, and compared the expression of miRNAs.
Results: RT-PCR array demonstrated 6 miRNAs (miR-32-5p, 885-5p, 665, 338-3p, 663a, 200a-3p) in apical part and 4 miRNAs (miR-32-5p, 665, 338-3p, 663a) from coronal part are significantly lower than molar group whereas miR-23a-3p is the only one that has significantly increase in both apical and coronal part compare to the molars group.
Conclusions: The results suggested that these miRNAs may have a role in mineralization process during prolonged crown formation of rodent incisors