IADR Abstract Archives
An Increase in BFGF Expression and Imbalanced Mitochondrial Dynamics are Observed in Human Pulpitis
Objectives: The objective of this study was to determine apoptosis, the balance of mitochondrial dynamics, and bFGF expression in human pulpitis in compared with healthy pulp.
Methods: Sixteen pulp tissues from healthy, as control, (n=8) and irreversible pulpitis (n=8) were collected from the Oral and Maxillofacial Surgery clinic, Faculty of Dentistry, Chiang Mai University. Apoptosis, mitochondrial dynamics, and bFGF expressions in pulpal tissues were determined by western blot analysis.
Results: In human pulpitis, there was a significant increase in apoptotic markers as indicated by increased Bax/Bcl2 ratio, when compared with those of controls (p<0.05). An imbalance of mitochondrial dynamics as indicated by decreased mitochondrial fusion proteins, Mfn2 and OPA1, and increased mitochondrial fission protein, Drp-1 were also observed in pulpitis, when compared with those of controls (p<0.05). In addition, the expression of bFGF was significantly increased in pulpitis, when compared with that of control (p<0.05). bFGF also showed positive correlations with cell apoptosis (r=0.5484, p<0.05) and mitochondrial fission (r=0.8333, p<0.05).
Conclusions: These findings suggest that increased apoptosis and an imbalance of mitochondrial dynamics occur in irreversible pulpitis, but factors for wound healing in pulpitis as bFGF up-regulates. An increase in bFGF in pulpitis could be the process to maintain cell survivals in pulpal inflammation in order to compensate an increased cell apoptosis and mitochondrial fission events.
Methods: Sixteen pulp tissues from healthy, as control, (n=8) and irreversible pulpitis (n=8) were collected from the Oral and Maxillofacial Surgery clinic, Faculty of Dentistry, Chiang Mai University. Apoptosis, mitochondrial dynamics, and bFGF expressions in pulpal tissues were determined by western blot analysis.
Results: In human pulpitis, there was a significant increase in apoptotic markers as indicated by increased Bax/Bcl2 ratio, when compared with those of controls (p<0.05). An imbalance of mitochondrial dynamics as indicated by decreased mitochondrial fusion proteins, Mfn2 and OPA1, and increased mitochondrial fission protein, Drp-1 were also observed in pulpitis, when compared with those of controls (p<0.05). In addition, the expression of bFGF was significantly increased in pulpitis, when compared with that of control (p<0.05). bFGF also showed positive correlations with cell apoptosis (r=0.5484, p<0.05) and mitochondrial fission (r=0.8333, p<0.05).
Conclusions: These findings suggest that increased apoptosis and an imbalance of mitochondrial dynamics occur in irreversible pulpitis, but factors for wound healing in pulpitis as bFGF up-regulates. An increase in bFGF in pulpitis could be the process to maintain cell survivals in pulpal inflammation in order to compensate an increased cell apoptosis and mitochondrial fission events.