IADR Abstract Archives

Periostin Promoted PDL Cell Migration and Myofibroblast Differentiation

Objectives: Periostin is a matricellular protein involved in cell adhesion, migration, and modulating cellular response to mechanical stimulation. The exact function of periostin in periodontal tissue is unresolved. The aim of this study was to investigate the function of this protein on periodontal ligament (PDL) cells.
Methods: Human PDL cells were isolated from the periodontal tissue of 5 permanent molars. A scratch wound assay was used to investigate cell migration. Cells were subjected to intermittent compressive force (ICF) for 24 h and alpha-smooth muscle actin (α-SMA) and receptor activator of nuclear factor kappa-B ligand (RANKL) expression was examined using quantitative reverse transcription polymerase chain reaction and western blot. The periostin gene (POSTN) was knocked down by transfecting the cells with periostin siRNA. Statistical significance was considered at p < 0.05 (Anova test).
Results: Periostin-coated surfaces accelerated cell movement compared with the control in the scratch wound assay. Although the periostin-knockdown cells (POSTN-KD cells) migrated slower compared with control, the decreased migratory rate was rescued when the POSTN-KD cells were seeded on periostin-coated surfaces. RANKL expression increased in the POSTN-KD cells, however, the ICF-induced RANKL expression was attenuated in POSTN-KD cells. ICF also increased α-SMA expression, however, POSTN-KD cell responsiveness to ICF decreased significantly compared with the control. Interestingly, it is periostin-coated surfaces that reduced these ICF-inductive effects, not soluble form of periostin as in other previous report.
Conclusions: Periostin promoted PDL cell migration. Moreover, ICF promoted myofibroblast differentiation in PDL cells based on the significantly increased α-SMA expression (p < 0.05) compared with the control. ICF regulated α-SMA and RANKL is a periostin dependent. However, the impact of ICF on RANKL expression remains unclear. This finding emphasizes the importance of extracellular matrix proteins on PDL cell function.

2020 South East Asia Division Meeting (Virtual)

2020
S010
Pulp Biology & Regeneration Research
  • Manosuttikij, Benjaporn  ( Chulalongkorn University , Bangkok , Thailand )
  • Panjasiri, Natchanan  ( Chulalongkorn University , Bangkok , Thailand )
  • Putta, Chanisara  ( Chulalongkorn University , Bangkok , Thailand )
  • Pavasant, Prasit  ( Chulalongkorn University , Bangkok , Thailand )
  • NONE
    Dental Research Fund, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.: Dental research project 3200502#1/2016.
    Oral Session
    Junior Hatton
    Thursday, 11/26/2020 , 03:15PM - 05:00PM