IADR Abstract Archives
In-Vitro Antibacterial Efficacy of Methanolic Extract of Vitex Parviflora a. Juss Leaves Against Streptococcus Mutans
Objectives: Studies have shown antibacterial activities of methanolic crude extracts of Vitex parviflora A. Juss (molave) leaves and stem against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Bacillus subtilis but, has not been tested against oral pathogens. This study determined the antibacterial efficacy of molave leaves extract on growth inhibition of Streptococcus mutans.
Methods: An in vitro experimental design was used to determine the antibacterial efficacy of molave leaves extract on the inhibition of Streptococcus mutans through disk diffusion method. For cost-efficiency, total sample size of 80 plates were reduced by placing 5 test groups in one plate divided in 5 portions. The test was done in 18 replicates. From a single tree, 500 grams of fresh molave leaves were collected and subjected to a methanolic extraction process. Molave extracts in 50 mg/ml and 100 mg/ml concentrations, 0.12% chlorhexidine, distilled water, and 95% methanol were tested on 18 Mueller-Hinton agar (MHA) plates inoculated with Streptococcus mutans. Plates were incubated for 48 hours in anaerobic conditions and resulting zones of inhibition of each solution were measured with a millimeter ruler. Data were then analyzed through one-way ANOVA and Bonferroni tests.
Results: Mean diameter of zones of inhibition produced by 100 mg/ml and, 50 mg/ml concentrations of Vitex parviflora A. juss methanolic leaves extract, and 0.12% chlorhexidine were 15.78mm, 11.63 mm, and 21.44mm respectively. Both distilled water and 95% methanol did not inhibit bacterial growth. Results suggest that 100 mg/ml concentration has stronger antibacterial properties than 50 mg/ml concentration. Furthermore, both concentrations of molave leaves extract were relatively weaker compared to chlorhexidine.
Conclusions: Vitex parviflora A. juss methanolic leaves extract have the ability to inhibit the growth of Streptococcus mutans, under ideal circumstances, based on the diameter of zone of inhibition through disk diffusion method.
Methods: An in vitro experimental design was used to determine the antibacterial efficacy of molave leaves extract on the inhibition of Streptococcus mutans through disk diffusion method. For cost-efficiency, total sample size of 80 plates were reduced by placing 5 test groups in one plate divided in 5 portions. The test was done in 18 replicates. From a single tree, 500 grams of fresh molave leaves were collected and subjected to a methanolic extraction process. Molave extracts in 50 mg/ml and 100 mg/ml concentrations, 0.12% chlorhexidine, distilled water, and 95% methanol were tested on 18 Mueller-Hinton agar (MHA) plates inoculated with Streptococcus mutans. Plates were incubated for 48 hours in anaerobic conditions and resulting zones of inhibition of each solution were measured with a millimeter ruler. Data were then analyzed through one-way ANOVA and Bonferroni tests.
Results: Mean diameter of zones of inhibition produced by 100 mg/ml and, 50 mg/ml concentrations of Vitex parviflora A. juss methanolic leaves extract, and 0.12% chlorhexidine were 15.78mm, 11.63 mm, and 21.44mm respectively. Both distilled water and 95% methanol did not inhibit bacterial growth. Results suggest that 100 mg/ml concentration has stronger antibacterial properties than 50 mg/ml concentration. Furthermore, both concentrations of molave leaves extract were relatively weaker compared to chlorhexidine.
Conclusions: Vitex parviflora A. juss methanolic leaves extract have the ability to inhibit the growth of Streptococcus mutans, under ideal circumstances, based on the diameter of zone of inhibition through disk diffusion method.