IADR Abstract Archives

Iloprost Treatment Induced Bone and Cementum Formation in an Immediate rat Tooth Avulsion Model

Objectives: The aim of the present study was to evaluate the influence of root surface pretreatment with iloprost on the histomorphometric parameters of rat molars after immediate replantation.
Methods: Tooth replantation was performed on the maxillary molars of 8 weeks-old Wistar rats. The teeth were extracted and the root surfaces were treated with iloprost or Hanks’ balanced salt solution (HBSS) prior to replantation. The animals were sacrificed after 30 days. Histological sections of the specimens were prepared and underwent hematoxylin and eosin (H&E) and tartrate-resistant acid phosphatase (TRACP) staining. Histological analyses were performed; bone volume (BV/TV), osteoclast surface (OC.S/BS), osteoclast volume density (N.Oc/T.Ar), osteoclast bone surface density (N.Oc/B.Pm), acellular cementum, and inflammatory score (IS). The results were analyzed using the Mann-Whitney test at the 95% significance level.
Results: Root and bone resorption,and inflammation occurred in both groups. The histomorphometric parameters; OC.S/BS, N.Oc/T.Ar, N.Oc/B.Pm, and IS were not significantly different between the iloprost-treated and the control groups. However, BV/TV was higher in the iloprost-treated group compared with the control HBSS group. Acellular cementum was observed in some specimens in the iloprost-treated group and the acellular cementum was thicker in the apical area compared with the coronal area.
Conclusions: The results demonstrated reduced root resorption and inflammation in the iloprost-treated group. Moreover, the increased bone volume and acellular cementum in the iloprost group indicate that iloprost may promote the healing process. These data suggest that iloprost could be further used to reduce root/bone resorption in clinical tooth replantation.

2020 South East Asia Division Meeting (Virtual)

2020
S004
Mineralized Tissue
  • Chenboonthai, Nichakorn  ( Chulalongkorn University , Bangkok , Thailand )
  • Nisaeh, Natnicha  ( Chulalongkorn University , Bangkok , Thailand )
  • Teantongdee, Araya  ( Chulalongkorn University , Bangkok , Thailand )
  • Yodsanga, Somchai  ( Chulalongkorn University , Bangkok , Thailand )
  • Osathanon, Thanaphum  ( Chulalongkorn University , Bangkok , Thailand )
  • Limjeerajarus, Chalida  ( Chulalongkorn University , Bangkok , Thailand )
  • NONE
    Oral Session
    Junior Hatton
    Thursday, 11/26/2020 , 03:15PM - 05:00PM