IADR Abstract Archives
Remineralization Potential of Arginine-Fluoride Varnish in a Bacterial pH-Cycling Model.
Objectives: To examine the enamel fluoride uptake and remineralization potential of arginine-fluoride (Arg-NaF) varnishes in a simulated clinical condition using a multi-species bacterial pH-cycling model.
Methods: L-Arginine (at 1%, 2%, and 4% by wt.) was incorporated in a 5% NaF varnish. Experimental and control groups were: 1% Arg-NaF; 2% Arg-NaF; 4% Arg-NaF; NaF; and no treatment. Artificial incipient caries-like lesions were formed on 30 enamel specimen blocks (n=6). The specimens underwent multi-species bacterial pH-cycling in an artificial mouth system using oral biofilm reactor for 72 h after treatment with experimental and control varnishes. The multi-species bacterial pH-cycling model included Streptococcus mutans, Streptococcus sanguinis, and Streptococcus gordonii at 1:1:1 ratio in BHI broth (pH-5.0) with 0.5% yeast extract, 2% sucrose, and 1% glucose which served as a suspension for demineralization. For remineralization period, the specimens were subjected to artificial saliva flow in the biofilm reactor. After bacterial pH-cycling, the specimens were evaluated for mineral density using micro-CT, Ca/P ratio with SEM-EDX, and enamel (EFU) with biofilm/plaque (PFU) fluoride uptake. The mineral gain, percentage remineralization, and percentage change in Ca/P were computed. The statistical analysis was done using 1-/2-way ANOVA with SNK/Bonferroni’s post-hoc test and the statistical significance was set at α=0.05.
Results: Increasing concentrations of Arg in NaF varnish significantly increased the EFU of incipient caries-like lesions (p<0.001). The PFU for 1% Arg-NaF was significantly higher than 4% Arg-NaF and the control NaF (p<0.05). Post pH-cycling, Ca/P ratio with 1%/2% Arg-NaF was closest to hydroxyapatite (1.67). Mineral gain and % remineralization of 1%/2% Arg-NaF were significantly higher than the control NaF varnish (p<0.05).
Conclusions: The prebiotic L-arginine (at 1%/2% by wt.) in a 5% NaF varnish enhanced the enamel fluoride uptake and remineralization potential of the conventional 5% NaF varnish.
Methods: L-Arginine (at 1%, 2%, and 4% by wt.) was incorporated in a 5% NaF varnish. Experimental and control groups were: 1% Arg-NaF; 2% Arg-NaF; 4% Arg-NaF; NaF; and no treatment. Artificial incipient caries-like lesions were formed on 30 enamel specimen blocks (n=6). The specimens underwent multi-species bacterial pH-cycling in an artificial mouth system using oral biofilm reactor for 72 h after treatment with experimental and control varnishes. The multi-species bacterial pH-cycling model included Streptococcus mutans, Streptococcus sanguinis, and Streptococcus gordonii at 1:1:1 ratio in BHI broth (pH-5.0) with 0.5% yeast extract, 2% sucrose, and 1% glucose which served as a suspension for demineralization. For remineralization period, the specimens were subjected to artificial saliva flow in the biofilm reactor. After bacterial pH-cycling, the specimens were evaluated for mineral density using micro-CT, Ca/P ratio with SEM-EDX, and enamel (EFU) with biofilm/plaque (PFU) fluoride uptake. The mineral gain, percentage remineralization, and percentage change in Ca/P were computed. The statistical analysis was done using 1-/2-way ANOVA with SNK/Bonferroni’s post-hoc test and the statistical significance was set at α=0.05.
Results: Increasing concentrations of Arg in NaF varnish significantly increased the EFU of incipient caries-like lesions (p<0.001). The PFU for 1% Arg-NaF was significantly higher than 4% Arg-NaF and the control NaF (p<0.05). Post pH-cycling, Ca/P ratio with 1%/2% Arg-NaF was closest to hydroxyapatite (1.67). Mineral gain and % remineralization of 1%/2% Arg-NaF were significantly higher than the control NaF varnish (p<0.05).
Conclusions: The prebiotic L-arginine (at 1%/2% by wt.) in a 5% NaF varnish enhanced the enamel fluoride uptake and remineralization potential of the conventional 5% NaF varnish.