IADR Abstract Archives

Non-Canonical Wnt Signaling Participates in Jagged1-Induced Odonto/Osteogenic Differentiation in HDPs

Objectives: To examine the involvement of Wnt signaling in Jagged1-induced odonto/osteogenic differentiation in human dental pulp cells (hDPs)
Methods: RNA sequencing data of Jagged1-treated hDPs was analyzed to identify their gene expression profile. The mRNA expression of HES1, HEY1, WNT2B, WNT5A, WNT5B, WNT16, DKK1, DKK2, and SOST was examined using real-time polymerase chain reaction. Osteogenic differentiation was determined using osteogenic-related gene expression, alkaline phosphatase (ALP) activity, and mineralization. Wnt signaling was activated using recombinant Wnt proteins, while Wnt signaling was inhibited using DKK1 or IWP-2
Results: Notch signaling activation using immobilized-Jagged1 enhanced odonto/osteogenic differentiation in hDPs as determined by increased ALP activity and mineralization. Bioinformatic analysis of the gene expression profile by high throughput RNA sequencing of the Jagged1-treated hDPs revealed that Notch regulated numerous Wnt-related genes. Among Wnt ligands, WNT2B and WNT5A mRNA levels were upregulated after Jagged1 treatment. In contrast, Wnt inhibitors, DKK1, DKK2, and SOST mRNA levels were downregulated in Jagged1-treated cells. Recombinant WNT2B and WNT5A significantly promoted in vitro mineral deposition by hDPs. The Wnt signaling inhibition experiments revealed that IWP-2, but not DKK1, inhibited Jagged1-induced ALP activity and mineralization
Conclusions: The Notch ligand, Jagged1, promoted odonto/osteogenic differentiation in hDPs by regulating the non-canonical Wnt pathway

2020 South East Asia Division Meeting (Virtual)

2020
P030
Pulp Biology & Regeneration Research
  • Kornsuthisopon, Chatvadee  ( Chulalongkorn University , Bangkok , Thailand ;  Chulalongkorn University , Bangkok , Thailand )
  • Manokawinchoke, Jeeranun  ( Chulalongkorn University , Bangkok , Thailand )
  • Tompkins, Kevin A.  ( Chulalongkorn University , Bangkok , Thailand )
  • Osathanon, Thanaphum  ( Chulalongkorn University , Bangkok , Thailand ;  Chulalongkorn University , Bangkok , Thailand )
  • NONE
    Poster Session
    Poster 3 (Senior Hatton)
    Thursday, 11/26/2020 , 02:30PM - 03:00PM