IADR Abstract Archives
Resistance of P.Gingivalis, S.Aureus, P.Aeruginosa to a Cetyl Pyridinium Chloride-Containing Mouthwash.
Objectives: To evaluate the bactericide effect of a Cetyl Pyridinium (CPC), Tranexamic Acid (TXA), and Dipotassium Glycyrrhizinate(Gk2)-based mouthwash in P.gingivalis, Staphylococcus aureus sp.,Pseudomonas Aureuginosa sp. all being microorganisms known for its high pathogenecity and drug-resistance.
Methods: Porphyromonas gingivalis (ATCC® 33277TM) (P.gingivalis), Staphylococcus aureus subbsp.aureus
KWIK-STIKTM (ATCC® 29213TM) (S.aureus), and Pseudomonas aeruginosa KWIK-STIKTM
(ATCC® 27853TM) (P.aeruginosa) were evaluated in presence or absence of CPC, TXA, and Gk2(+) or CPC, TXA, and Gk2(-) in
vitro. Solutions containing S.aureus and P.aeruginosa were plated into BD Trypticase Soy Agar II with
5% Sheep Blood (BD Diagnostics), and results were analyzed after 48 hours of aerobic incubation.
Whereas for P.gingivalis, bacterial solutions were plated in Brucella HK agar (Kyokuto
PharmaceuticalIndustrial Co.,Ltd.) and results were analyzed after 96 hours of anaerobic incubation
which was achieved by Mitsubishi™ AnaeroPacks, all procedures were repeated 4 times. A
quantitative analysis was performed with ImageJ (version 2.0.0) in order to measure the amount of
surface colonization of the plates by bacteria. The p-value was evaluated with an unpaired F test to
compare variances (Prism8 for MacOS). Results with a p-value <0.05 were considered as statistically
significant.
Results: The bactericide effect in Cpc, Gk2, TXA (+) was evidentiably total for P.gingivalis, and significant for
S.aureus and P.aeruginosa compared to plates belonging to Cpc, Gk2, TXA (-).
Conclusions: Surface colonization by P.gingivalis, S.aureus, and P.aeruginosa was inhibited by a CPC, Gk2 and
TXA-containing solution. Taken altogether, these results show that a mouthwash containing these
agents, might inhibibt the proliferation of these bacteria.
Methods: Porphyromonas gingivalis (ATCC® 33277TM) (P.gingivalis), Staphylococcus aureus subbsp.aureus
KWIK-STIKTM (ATCC® 29213TM) (S.aureus), and Pseudomonas aeruginosa KWIK-STIKTM
(ATCC® 27853TM) (P.aeruginosa) were evaluated in presence or absence of CPC, TXA, and Gk2(+) or CPC, TXA, and Gk2(-) in
vitro. Solutions containing S.aureus and P.aeruginosa were plated into BD Trypticase Soy Agar II with
5% Sheep Blood (BD Diagnostics), and results were analyzed after 48 hours of aerobic incubation.
Whereas for P.gingivalis, bacterial solutions were plated in Brucella HK agar (Kyokuto
PharmaceuticalIndustrial Co.,Ltd.) and results were analyzed after 96 hours of anaerobic incubation
which was achieved by Mitsubishi™ AnaeroPacks, all procedures were repeated 4 times. A
quantitative analysis was performed with ImageJ (version 2.0.0) in order to measure the amount of
surface colonization of the plates by bacteria. The p-value was evaluated with an unpaired F test to
compare variances (Prism8 for MacOS). Results with a p-value <0.05 were considered as statistically
significant.
Results: The bactericide effect in Cpc, Gk2, TXA (+) was evidentiably total for P.gingivalis, and significant for
S.aureus and P.aeruginosa compared to plates belonging to Cpc, Gk2, TXA (-).
Conclusions: Surface colonization by P.gingivalis, S.aureus, and P.aeruginosa was inhibited by a CPC, Gk2 and
TXA-containing solution. Taken altogether, these results show that a mouthwash containing these
agents, might inhibibt the proliferation of these bacteria.