Macrophages Analysis on Gingival Tissue of Diabetic Rats after Insulin Leaf Extract Administration

Objectives: To determine the effect of insulin leaf extract to decrease the number of macrophages in the gingival tissues of diabetic rats that induced A. actinomycetemcomitans.
Methods: This study was laboratory experimental with post-test only. Samples were divided into two groups; treatment group and control group. The control group rats were given alloxan and A. actinomycetemcomitans, while the experimental group were given alloxan, A. actinomycetemcomitans, and the insulin leaf extract. Examination of macrophages is done by making histological preparations with Hematoxin-eosin staining.
Results: The mean number of macrophages in control group was 6.04±2.133, while in the treatment group was 2.62±0.517. Statistical analytic using Paired T-test showed a significant decrease in the number of macrophages (p<0.049).
Conclusions: There was a decrease in the number of macrophages in the gingival tissues of diabetic rats induced by A. actinomycetemcomitans after giving insulin leaf extract.